Detailed information of TA system TA149952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2122837..2123452
• Replicon: chromosome
• Accession: NZ_CP048751
• Organism: Brevundimonas mediterranea strain D151-2-6

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B4WDE8
• Locus tag: GYM46_RS10435
• Protein ID: WP_008258638.1
• Coordinates: 2122837..2123151 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GYM46_RS10440
• Protein ID: WP_008262856.1
• Coordinates: 2123144..2123452 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYM46_RS10415	2119476..2120552	-	1077	WP_008259793.1	mechanosensitive ion channel	-
GYM46_RS10425	2120930..2121388	-	459	WP_008261678.1	TIGR02300 family protein	-
GYM46_RS10430	2121513..2122823	+	1311	WP_008264235.1	3-phosphoshikimate 1-carboxyvinyltransferase	-
GYM46_RS10435	2122837..2123151	+	315	WP_008258638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GYM46_RS10440	2123144..2123452	+	309	WP_008262856.1	putative addiction module antidote protein	Antitoxin
GYM46_RS10445	2123452..2124096	+	645	WP_008262509.1	(d)CMP kinase	-
GYM46_RS10450	2124291..2125994	+	1704	WP_008262491.1	30S ribosomal protein S1	-
GYM46_RS10455	2126240..2126530	+	291	WP_035307045.1	integration host factor subunit beta	-
GYM46_RS10460	2126565..2126981	+	417	WP_008263083.1	large-conductance mechanosensitive channel protein MscL	-
GYM46_RS10465	2127011..2128159	-	1149	WP_008262218.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (12-101)

Antitoxin

• Uncharacterized protein HI1420 (5-94)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11890.43 Da
• Isoelectric Point: 9.4789

>T149952 WP_008258638.1 NZ_CP048751:2122837-2123151 [Brevundimonas mediterranea]
MDDNAAVRLTFTPVFDRWLLRFRDHRAVARITTRLERLSGGHWGDVKPVGEGVSELRIAYGPGYRLYATKKNDAWVILLA
GGDKSSQDRDIVEAKRLAEEYRND

Nucleotide

• Length: 315 bp

>T149952 NZ_CP064352:c4047230-4046847 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11169.75 Da
• Isoelectric Point: 4.3895

>AT149952 WP_008262856.1 NZ_CP048751:2123144-2123452 [Brevundimonas mediterranea]
MTEPLIEFDPARYLNTPEAVEEYLVSSFEDAAELGDPRIITNALGVVARAKGMTQMARDADLSRTALYRALSEEGRPELS
TIFKVMQALGLRLAPVRVDSAA

Nucleotide

• Length: 309 bp

>AT149952 NZ_CP064352:c4047472-4047230 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: B4WDE8