Detailed information of TA system TA149948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 712114..712763
• Replicon: chromosome
• Accession: NZ_CP048751
• Organism: Brevundimonas mediterranea strain D151-2-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GYM46_RS03545
• Protein ID: WP_008261384.1
• Coordinates: 712114..712503 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B4WA87
• Locus tag: GYM46_RS03550
• Protein ID: WP_008262398.1
• Coordinates: 712503..712763 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYM46_RS03500	707162..707392	+	231	WP_008262274.1	hypothetical protein	-
GYM46_RS03505	707426..708088	+	663	WP_040349274.1	phosphoribosylformylglycinamidine synthase subunit PurQ	-
GYM46_RS03510	708234..708971	+	738	WP_156796402.1	hypothetical protein	-
GYM46_RS03515	708988..710074	-	1087	Protein_687	aspartyl/asparaginyl beta-hydroxylase domain-containing protein	-
GYM46_RS03520	710118..710300	-	183	WP_154726307.1	hypothetical protein	-
GYM46_RS03525	710356..710748	-	393	WP_008263920.1	hypothetical protein	-
GYM46_RS03530	710872..711663	+	792	WP_008262651.1	hypothetical protein	-
GYM46_RS03535	711660..711911	-	252	WP_008259943.1	hypothetical protein	-
GYM46_RS03540	711911..712054	-	144	WP_008260671.1	hypothetical protein	-
GYM46_RS03545	712114..712503	-	390	WP_008261384.1	type II toxin-antitoxin system VapC family toxin	Toxin
GYM46_RS03550	712503..712763	-	261	WP_008262398.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GYM46_RS03555	712831..715047	+	2217	WP_008259717.1	phosphoribosylformylglycinamidine synthase subunit PurL	-
GYM46_RS03560	715026..715397	+	372	WP_156796517.1	DUF305 domain-containing protein	-
GYM46_RS03565	715400..715903	+	504	WP_035308435.1	hypothetical protein	-
GYM46_RS03570	716001..717389	+	1389	WP_008261181.1	cytochrome P450	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-85)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14311.45 Da
• Isoelectric Point: 5.2477

>T149948 WP_008261384.1 NZ_CP048751:c712503-712114 [Brevundimonas mediterranea]
MVVDTSAFVAIVLREPEAAAFDERIRSATTRRVSVATWAELAAVLTSRLGVDARPVLDRLEQEYRLDLVPVDQAQMRIAC
EAMVRFGKGRRPARLNYGDCFAYALSLSSSEPLLFKGEDFSKTDVAITI

Nucleotide

• Length: 390 bp

>T149948 NZ_CP064352:2813095-2813313 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9600.81 Da
• Isoelectric Point: 4.7473

>AT149948 WP_008262398.1 NZ_CP048751:c712763-712503 [Brevundimonas mediterranea]
MGLSIKRAETERKARAVAERLGVSLTEAIDIALDKTWKELTAEEGAAERASKREALFAYLRTLPPGDGRSLQEVDDEMYD
EHGLPR

Nucleotide

• Length: 261 bp

>AT149948 NZ_CP064352:2812694-2813068 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGCCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: B4WA87