Detailed information of TA system TA149947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 699682..700204
• Replicon: chromosome
• Accession: NZ_CP048751
• Organism: Brevundimonas mediterranea strain D151-2-6

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: B4WAA9
• Locus tag: GYM46_RS03450
• Protein ID: WP_008261032.1
• Coordinates: 699917..700204 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4WAB0
• Locus tag: GYM46_RS03445
• Protein ID: WP_008261500.1
• Coordinates: 699682..699927 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYM46_RS03425	695575..695850	-	276	WP_040349276.1	DUF2849 domain-containing protein	-
GYM46_RS03430	695847..697232	-	1386	WP_008263762.1	siroheme synthase CysG	-
GYM46_RS03435	697357..698286	+	930	WP_008261515.1	transcriptional regulator GcvA	-
GYM46_RS03440	698337..699638	+	1302	WP_008264378.1	adenylosuccinate lyase	-
GYM46_RS03445	699682..699927	+	246	WP_008261500.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GYM46_RS03450	699917..700204	+	288	WP_008261032.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GYM46_RS03455	700201..700704	+	504	WP_008258747.1	DNA-deoxyinosine glycosylase	-
GYM46_RS03460	700704..701546	+	843	WP_008261825.1	formyltetrahydrofolate deformylase	-
GYM46_RS03465	701653..702282	+	630	WP_008261651.1	fasciclin domain-containing protein	-
GYM46_RS03470	702467..702790	-	324	WP_196066380.1	DUF1476 domain-containing protein	-
GYM46_RS03475	703076..703834	+	759	WP_008263270.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GYM46_RS03480	703834..704070	+	237	WP_008260221.1	phosphoribosylformylglycinamidine synthase subunit PurS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-71)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11165.94 Da
• Isoelectric Point: 10.4934

>T149947 WP_008261032.1 NZ_CP048751:699917-700204 [Brevundimonas mediterranea]
MPFDLQFLPSALKEWEKLGAPVRAQFKTKLAERLEYPRVPKDALHGMRDHYKIKLRDAGYRLVYRVEDAIVTVTVVAVGR
RDRSQVYRDAARRHE

Nucleotide

• Length: 288 bp

>T149947 NZ_CP064352:c2687767-2687450 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8829.11 Da
• Isoelectric Point: 4.4865

>AT149947 WP_008261500.1 NZ_CP048751:699682-699927 [Brevundimonas mediterranea]
MQRVEASVAVSVSDLKKSPSAVMDDAAGQTVAVLNHNRVMAYMVPAAAYEAMVERLDDLALIETIRQRQDETPVRVSLDA
L

Nucleotide

• Length: 246 bp

>AT149947 NZ_CP064352:c2687457-2687170 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: B4WAA9

Antitoxin

• AlphaFold DB: B4WAB0