Detailed information of TA system TA149936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1688214..1689011
• Replicon: chromosome
• Accession: NZ_CP048736
• Organism: Enterobacter sp. T2

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: G0034_RS07950
• Protein ID: WP_176450254.1
• Coordinates: 1688487..1689011 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A2W0G3H3
• Locus tag: G0034_RS07945
• Protein ID: WP_014883303.1
• Coordinates: 1688214..1688483 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0034_RS07915 (1683834)	1683834..1684334	+	501	WP_014883299.1	DedA family protein	-
G0034_RS07920 (1684438)	1684438..1685679	+	1242	WP_023337066.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
G0034_RS07925 (1685714)	1685714..1685941	-	228	WP_014883300.1	YccJ family protein	-
G0034_RS07930 (1685960)	1685960..1686556	-	597	WP_014883301.1	NAD(P)H:quinone oxidoreductase	-
G0034_RS07935 (1686948)	1686948..1687118	+	171	WP_023332656.1	general stress protein	-
G0034_RS07940 (1687235)	1687235..1688152	+	918	WP_047028038.1	DMT family transporter	-
G0034_RS07945 (1688214)	1688214..1688483	+	270	WP_014883303.1	DUF1778 domain-containing protein	Antitoxin
G0034_RS07950 (1688487)	1688487..1689011	+	525	WP_176450254.1	GNAT family N-acetyltransferase	Toxin
G0034_RS07955 (1689085)	1689085..1690407	-	1323	WP_179120698.1	pyrimidine utilization transport protein G	-
G0034_RS07960 (1690429)	1690429..1690923	-	495	WP_069601907.1	pyrimidine utilization flavin reductase protein F	-
G0034_RS07965 (1690933)	1690933..1691523	-	591	WP_014883307.1	malonic semialdehyde reductase	-
G0034_RS07970 (1691533)	1691533..1692333	-	801	WP_069601908.1	pyrimidine utilization protein D	-
G0034_RS07975 (1692341)	1692341..1692727	-	387	WP_014883309.1	pyrimidine utilization protein C	-
G0034_RS07980 (1692739)	1692739..1693428	-	690	WP_014883310.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19689.59 Da
• Isoelectric Point: 8.9464

>T149936 WP_176450254.1 NZ_CP048736:1688487-1689011 [Enterobacter sp. T2]
IVANLTIEMFSEGSDYDFRGFDCGEPSLNAFLTEHLVRQHKGRILRGYLLKDRDHPRVLGYYTLSGSCFEKAMLPSKTQQ
RRIPYVNVPSVTLGRLAVHKDLQGKEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNEQAKRFYLKQGFIPLTGENSHSL
FFPTKSIEQLFEQA

Nucleotide

• Length: 525 bp

>T149936 NZ_CP064351:481650-481982 [Vibrio cholerae C6706]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10278.72 Da
• Isoelectric Point: 5.8836

>AT149936 WP_014883303.1 NZ_CP048736:1688214-1688483 [Enterobacter sp. T2]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNESSWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT149936 NZ_CP064351:481969-482283 [Vibrio cholerae C6706]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGTGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACACATAGCCGAGCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2W0G3H3