Detailed information of TA system TA149929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-doc
• Location: 5613..6241
• Replicon: plasmid pNW26-2
• Accession: NZ_CP048735
• Organism: Mammaliicoccus sciuri strain NWAF26

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: G3T51_RS14215
• Protein ID: WP_152292094.1
• Coordinates: 5613..6008 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: G3T51_RS14220
• Protein ID: WP_172965111.1
• Coordinates: 6005..6241 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3T51_RS14185	1330..1764	-	435	WP_152292097.1	RusA family crossover junction endodeoxyribonuclease	-
G3T51_RS14190	1782..2240	-	459	WP_152292096.1	hypothetical protein	-
G3T51_RS14195	2750..3025	-	276	WP_058592528.1	hypothetical protein	-
G3T51_RS14200	3046..3843	-	798	WP_058592527.1	ParA family protein	-
G3T51_RS14205	4428..4586	-	159	WP_172965110.1	hypothetical protein	-
G3T51_RS14210	4783..5475	-	693	WP_152292095.1	hypothetical protein	-
G3T51_RS14215	5613..6008	-	396	WP_152292094.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
G3T51_RS14220	6005..6241	-	237	WP_172965111.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G3T51_RS14225	6734..7180	-	447	WP_152292092.1	GNAT family N-acetyltransferase	-
G3T51_RS14230	7193..7612	-	420	WP_152292091.1	FosB/FosD family fosfomycin resistance bacillithiol transferase	-
G3T51_RS14235	7927..8070	-	144	WP_172965109.1	hypothetical protein	-
G3T51_RS14240	8079..8309	-	231	WP_185160637.1	hypothetical protein	-
G3T51_RS14245	8346..8558	-	213	WP_152292089.1	XRE family transcriptional regulator	-
G3T51_RS14250	8649..8867	-	219	WP_152292088.1	hypothetical protein	-
G3T51_RS14255	8902..9126	-	225	WP_152292087.1	hypothetical protein	-
G3T51_RS14260	9119..9460	-	342	WP_152292086.1	hypothetical protein	-
G3T51_RS14265	9515..9688	-	174	WP_172965108.1	hypothetical protein	-
G3T51_RS14270	9742..10248	-	507	WP_152292085.1	hypothetical protein	-
G3T51_RS14275	10486..10632	-	147	WP_172965107.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	fosD	-	1..100455	100455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-127)

Antitoxin

• SpoVT-AbrB domain (8-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15016.54 Da
• Isoelectric Point: 9.4962

>T149929 WP_152292094.1 NZ_CP048735:c6008-5613 [Mammaliicoccus sciuri]
IMLYLTEKDIITLNIYVINKYSPKEQIGVKEPTALNMLVNAPKQHVFGQEQYPTIQLKAANLVRNLVKKHVFHNGNKRTA
FMALIVFLELNGYKFVVSNKEAVNYMVKLATDDINEDEIALWISAYSQLSK

Nucleotide

• Length: 396 bp

>T149929 NZ_CP064351:c395445-395176 [Vibrio cholerae C6706]
ATGTATAAACTCGAATACTCCACACAGTTTAAGAAAGATTTCAAAAAGATAACTAAGATGCCGATTTCAGACATCATTGA
AGTCGGCAATGTTATTTCTAAGCTGCAACGCGGCGAAAAGCTTGAACCCAAGAATGTTGACCATCCGTTAACTGGAAATT
GGGTTGGATTTCGAGACTGCCACATTAAACCCGACCTAGTGTTAATCTATCGAGTTTTTAACGATCAACTACAACTAGCG
CGCATTGGTTCACATAGTGATTTATTCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8677.86 Da
• Isoelectric Point: 6.2988

>AT149929 WP_172965111.1 NZ_CP048735:c6241-6005 [Mammaliicoccus sciuri]
VINTRKLRKIGNSRVVTVPEEVIKELKISDGQKISFNIDNGKVIIEAVDNGNQGTDILSIAERVSNQYNQALQELVDK

Nucleotide

• Length: 237 bp

>AT149929 NZ_CP064351:c395716-395438 [Vibrio cholerae C6706]
ATGAGAACAGAAATGCTGAGCACGCGCATTGACCACGACACGAAAATTGCGTTCACAAACGTCTGCGATGAGATGGGTCT
AAGTACATCACAGGCCATAAAGTTATTTGCAAAAGCGGTTATTAATCATGGTGGAATCCCTTTTGAGCTGCGAGTTCCAC
AGCCTAATGAGGTTACTGCATCTGCAATTCAAGAGCTCGTTGAAGGAAAAGGACATAAAGCTGAATCCGTTGAAGCTATG
CTGAATGAGCTTACTGAAGGCAAAGTTAAGCATGTATAA