Detailed information of TA system TA149927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1069621..1070160
• Replicon: chromosome
• Accession: NZ_CP048732
• Organism: Mammaliicoccus sciuri strain NWAF26

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A418JBZ2
• Locus tag: G3T51_RS05185
• Protein ID: WP_048540867.1
• Coordinates: 1069789..1070160 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G3T51_RS05180
• Protein ID: WP_048540868.1
• Coordinates: 1069621..1069788 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3T51_RS05155	1065503..1065982	+	480	WP_119493483.1	PH domain-containing protein	-
G3T51_RS05160	1065972..1067492	+	1521	WP_048540875.1	PH domain-containing protein	-
G3T51_RS05165	1067482..1067946	+	465	WP_075578106.1	PH domain-containing protein	-
G3T51_RS05170	1067978..1068340	+	363	WP_058591084.1	holo-ACP synthase	-
G3T51_RS05175	1068388..1069536	+	1149	WP_048540869.1	alanine racemase	-
G3T51_RS05180	1069621..1069788	+	168	WP_048540868.1	hypothetical protein	Antitoxin
G3T51_RS05185	1069789..1070160	+	372	WP_048540867.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G3T51_RS05190	1070257..1071261	+	1005	WP_048540866.1	PP2C family protein-serine/threonine phosphatase	-
G3T51_RS05195	1071366..1071689	+	324	WP_025904513.1	anti-sigma factor antagonist	-
G3T51_RS05200	1071694..1072170	+	477	WP_025904514.1	anti-sigma B factor RsbW	-
G3T51_RS05205	1072145..1072915	+	771	WP_037589755.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13653.83 Da
• Isoelectric Point: 9.9193

>T149927 WP_048540867.1 NZ_CP048732:1069789-1070160 [Mammaliicoccus sciuri]
MRRGDVYLADLSPVTGSEQGGTRPVVIIQNDTGNRYSPTVIVAAITGKINKAKIPTHVEIEAAKYKLDRDSVILLEQIRT
IDKKRLKEKLTYLSDAKMKEVDIAIAISLNLTLQQKFDTLGNT

Nucleotide

• Length: 372 bp

>T149927 NZ_CP064351:c390161-389844 [Vibrio cholerae C6706]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6120.58 Da
• Isoelectric Point: 4.0121

>AT149927 WP_048540868.1 NZ_CP048732:1069621-1069788 [Mammaliicoccus sciuri]
MTSLTQNRTQSLEQTLKEGYRSMADLNLSLASEAYYSECEACDSNESHLASQQGE

Nucleotide

• Length: 168 bp

>AT149927 NZ_CP064351:c390422-390180 [Vibrio cholerae C6706]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA

Structures

Toxin

• AlphaFold DB: A0A418JBZ2