Detailed information of TA system TA149924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 1076456..1076991
• Replicon: chromosome
• Accession: NZ_CP048713
• Organism: Staphylococcus lugdunensis strain CGMH-SL138

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: C5QLS6
• Locus tag: G3G86_RS05000
• Protein ID: WP_002445368.1
• Coordinates: 1076668..1076991 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: C5QLS5
• Locus tag: G3G86_RS04995
• Protein ID: WP_001058489.1
• Coordinates: 1076456..1076665 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3G86_RS04960	1071712..1071996	+	285	WP_002461410.1	co-chaperone GroES	-
G3G86_RS04965	1072056..1073678	+	1623	WP_002461409.1	chaperonin GroEL	-
G3G86_RS04970	1073747..1074919	-	1173	WP_002492309.1	tyrosine-type recombinase/integrase	-
G3G86_RS04975	1074933..1075607	-	675	WP_002445345.1	helix-turn-helix transcriptional regulator	-
G3G86_RS04980	1075780..1075998	+	219	WP_000153640.1	helix-turn-helix transcriptional regulator	-
G3G86_RS04985	1076003..1076320	+	318	WP_002445364.1	helix-turn-helix domain-containing protein	-
G3G86_RS04990	1076317..1076463	+	147	WP_002445365.1	hypothetical protein	-
G3G86_RS04995	1076456..1076665	+	210	WP_001058489.1	hypothetical protein	Antitoxin
G3G86_RS05000	1076668..1076991	+	324	WP_002445368.1	DUF1474 family protein	Toxin
G3G86_RS05005	1077055..1077924	+	870	WP_002445370.1	primase alpha helix C-terminal domain-containing protein	-
G3G86_RS05010	1077938..1079647	+	1710	WP_002445371.1	DUF927 domain-containing protein	-
G3G86_RS05015	1079995..1080360	+	366	WP_002445372.1	hypothetical protein	-
G3G86_RS05020	1080374..1081011	+	638	Protein_988	pathogenicity island protein	-
G3G86_RS05025	1081299..1081643	+	345	WP_094449183.1	pathogenicity island protein	-
G3G86_RS05030	1081657..1081890	+	234	WP_002492317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	1071712..1087123	15411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 13107.51 Da
• Isoelectric Point: 4.8571

>T149924 WP_002445368.1 NZ_CP048713:1076668-1076991 [Staphylococcus lugdunensis]
MNWEIKNLFSDLKLLKDSFEDLKDNHGWHFEELYPHEPNHNLNKDELIREGFSYHERRIHNNQMFDLFHLYIEQFDKIIQ
KFHELEKASSENFGEESDDAQKLKITE

Nucleotide

• Length: 324 bp

>T149924 NZ_CP064343:482188-482529 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8225.24 Da
• Isoelectric Point: 4.2820

>AT149924 WP_001058489.1 NZ_CP048713:1076456-1076665 [Staphylococcus lugdunensis]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVDGIEVVSEVSREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT149924 NZ_CP064343:481958-482188 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A853V3H1

Antitoxin

• AlphaFold DB: A0A853V601