Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 25335..25978 Replicon plasmid pEclE4-2
Accession NZ_CP048698
Organism Enterobacter hormaechei strain Eho-E4

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag GVI76_RS26665 Protein ID WP_000754566.1
Coordinates 25335..25751 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag GVI76_RS26670 Protein ID WP_001261276.1
Coordinates 25748..25978 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI76_RS26640 21685..22389 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
GVI76_RS26645 22452..22544 + 93 Protein_25 IS5/IS1182 family transposase -
GVI76_RS26650 22552..22860 - 309 Protein_26 DDE-type integrase/transposase/recombinase -
GVI76_RS26655 22877..23953 - 1077 WP_032448228.1 IS110 family transposase -
GVI76_RS26660 24235..25091 - 857 Protein_28 IS3-like element ISEc15 family transposase -
GVI76_RS26665 25335..25751 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
GVI76_RS26670 25748..25978 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GVI76_RS26675 26567..26860 + 294 WP_020956883.1 hypothetical protein -
GVI76_RS26680 26924..27610 + 687 WP_020956882.1 hypothetical protein -
GVI76_RS26685 27622..28398 + 777 WP_020956881.1 site-specific integrase -
GVI76_RS26690 28456..28713 - 258 WP_000764642.1 hypothetical protein -
GVI76_RS26695 28842..28946 - 105 WP_032409716.1 hypothetical protein -
GVI76_RS26700 29476..30342 + 867 WP_004118283.1 replication initiation protein -
GVI76_RS26705 30519..30788 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / blaVIM-1 / ant(3'')-Ia / qacE / sul1 / mph(A) galE / wza 1..96194 96194


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T149916 WP_000754566.1 NZ_CP048698:c25751-25335 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T149916 NZ_CP064334:1324018-1324299 [Xylella fastidiosa subsp. fastidiosa]
ATGCGGAGGATTAGCCAGACTGGCCAATTCAAGCGCGACTACAAGCGCGAAGCTAAGGGCCAGCATCGCGCCACGCTTGA
TGAGGAACTGATACACGTCTTGGAAGCCTTGACCTGTGATCATCCGTTGGAGCCACGCCACCACGATCACGCACTAACTG
GCGACTGGAAAGATCACCGTGATTGCCACATCAAACCCGACTTGGTACTGATATACCGGAAGCCCGACAACGAGACATTG
CAGCTCGTGCGCATTGGCTCACACAGCGAACTAGGTTTGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT149916 WP_001261276.1 NZ_CP048698:c25978-25748 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT149916 NZ_CP064334:1323768-1324031 [Xylella fastidiosa subsp. fastidiosa]
ATGTCTGCAAACGCAGTTGTCCGTGCCCGCGTTGACGCGCACATCAAAGAAGAAGCATCAACCGTGCTGGCGACGATGGG
CCTAAGCGTGTCTGATGCGTTCCGTATGATGCTGACCCGCATTGCCCGCGAGAAGGCGCTGCCGTTCGAGCCGCTGGTGC
CGAACGAAACTACCATCGCAGCCATGCGTGAAGCCCGTGCTGGTCACCTCAAGAGCTTTGATAGTATTGAGGCGCTCATG
GCGGACTTGCATGCGGAGGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References