Detailed information of TA system TA149896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 72523..73171
• Replicon: plasmid unnamed1
• Accession: NZ_CP048695
• Organism: Vibrio coralliilyticus OCN008

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: G3U99_RS25410
• Protein ID: WP_031342427.1
• Coordinates: 72523..72924 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: G3U99_RS25415
• Protein ID: WP_021455593.1
• Coordinates: 72938..73171 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3U99_RS25395	69019..69543	-	525	WP_085058120.1	MepB family protein	-
G3U99_RS25400	69783..70718	-	936	WP_021455596.1	bile acid:sodium symporter family protein	-
G3U99_RS25405	71286..72365	-	1080	Protein_66	ATP-binding protein	-
G3U99_RS25410	72523..72924	-	402	WP_031342427.1	type II toxin-antitoxin system VapC family toxin	Toxin
G3U99_RS25415	72938..73171	-	234	WP_021455593.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G3U99_RS25420	73426..74337	-	912	WP_021455592.1	LysR family transcriptional regulator	-
G3U99_RS25425	74445..75197	+	753	WP_165579941.1	SDR family oxidoreductase	-
G3U99_RS25430	75234..76133	-	900	WP_021455589.1	LysR family transcriptional regulator	-
G3U99_RS25435	76228..76950	+	723	WP_021455588.1	SDR family oxidoreductase	-
G3U99_RS25440	76965..77117	-	153	WP_021455587.1	hypothetical protein	-
G3U99_RS25445	77256..77663	-	408	WP_080568975.1	GFA family protein	-
G3U99_RS25450	77790..78077	+	288	WP_021455585.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..244689	244689

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14863.20 Da
• Isoelectric Point: 6.8538

>T149896 WP_031342427.1 NZ_CP048695:c72924-72523 [Vibrio coralliilyticus OCN008]
MLDTCICSFIMREQPITVLQKLQDVVGKQHRIVVSAITYQEMQYGLLGKKASPKHAVLVEEFLKRVDEILPWDKSAVDAT
TEVKRDLMAKGTPIGNNDTAIAGHAIATGCVLVTNNTREFQRVEGLKLEDWSH

Nucleotide

• Length: 402 bp

>T149896 NZ_CP064330:1388701-1389006 [Xylella fastidiosa subsp. fastidiosa]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9033.03 Da
• Isoelectric Point: 4.3324

>AT149896 WP_021455593.1 NZ_CP048695:c73171-72938 [Vibrio coralliilyticus OCN008]
MRTVSIFRNGKNQAVRLPKDFEFEGVNELEIHKEGDVITLRPVRPDWLSFGEQEKADDDFMATREDVITDEGRFNFE

Nucleotide

• Length: 234 bp

>AT149896 NZ_CP064330:1388401-1388697 [Xylella fastidiosa subsp. fastidiosa]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG