Detailed information of TA system TA149887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3204407..3205428
• Replicon: chromosome
• Accession: NZ_CP048692
• Organism: Proteus mirabilis strain HNS2p

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A8E4FRK4
• Locus tag: G3T55_RS14920
• Protein ID: WP_017827532.1
• Coordinates: 3204859..3205428 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: G3T55_RS14915
• Protein ID: WP_004245528.1
• Coordinates: 3204407..3204859 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3T55_RS14895 (G3T55_14915)	3200193..3200723	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
G3T55_RS14900 (G3T55_14920)	3201040..3202005	+	966	WP_049195511.1	thiamine ABC transporter substrate binding subunit	-
G3T55_RS14905 (G3T55_14925)	3202005..3203585	+	1581	WP_104731821.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
G3T55_RS14910 (G3T55_14930)	3203578..3204279	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
G3T55_RS14915 (G3T55_14935)	3204407..3204859	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
G3T55_RS14920 (G3T55_14940)	3204859..3205428	+	570	WP_017827532.1	PIN domain-containing protein	Toxin
G3T55_RS14925 (G3T55_14945)	3205544..3207898	+	2355	WP_107337099.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21601.08 Da
• Isoelectric Point: 7.0180

>T149887 WP_017827532.1 NZ_CP048692:3204859-3205428 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T149887 NZ_CP064328:c1581414-1581160 [Xylella fastidiosa subsp. fastidiosa]
ATGAAGCGTAAGCACGCTAAAACTCTCAGCGCTATATACACACGCCCTGTTTCGGCCAACATTCAATGGCGCGATATTGA
AGCGCTGTTCGTGGAGCTTGGAGCGAAAGTAGAAGAACGTGAAGGTTCTCGTATGTTGGTGCGACTGTTCGGTGAACGTC
GGATATTCCACCGGCCCCACCCTTCTCCTAACACTGACAAGGGTGCTGTTGAATCAATCCGAGCTTGGTTGAAATCGAAC
GGAGTTACACCATGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT149887 WP_004245528.1 NZ_CP048692:3204407-3204859 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT149887 NZ_CP064328:c1581163-1580828 [Xylella fastidiosa subsp. fastidiosa]
ATGAAGAACATCATGACTATTGAGAGCTTTAAAGCCATCATTACCTATGATCCGGAGATCGACATGTTTCGCGGTGAATT
TGTGGGTATCAATGGTGGTGCTGATTTCTATGCAAAAGACCTTAAGGGTCTTCGTCGAGAAGGAGCTATTTCTCTGAAGG
TATTTTTGGAAGCCTGCAAAGAAGATAGCGTGGAACCACGTAAATGCTACTCAGGGAAGTTCAATGCAAGGATTTCTCCT
GAACTGCATGCTTTGGCTTCTGAAGCCGCAGCAGCACAGGGCATCAGTTTGAACCAATTTGTAGAACAAGCGATTCAGCA
TGAAGTCCACACCTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3