Detailed information of TA system TA149883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 690825..691272
• Replicon: chromosome
• Accession: NZ_CP048686
• Organism: Lactococcus raffinolactis strain KACC 13441

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: G3T13_RS03320
• Protein ID: WP_082785381.1
• Coordinates: 691006..691272 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2A5SDT8
• Locus tag: G3T13_RS03315
• Protein ID: WP_061774359.1
• Coordinates: 690825..691016 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3T13_RS03305	686049..688004	-	1956	WP_096824040.1	DNA topoisomerase IV subunit B	-
G3T13_RS03310	688405..690558	-	2154	WP_061774358.1	type I DNA topoisomerase	-
G3T13_RS03315	690825..691016	+	192	WP_061774359.1	hypothetical protein	Antitoxin
G3T13_RS03320	691006..691272	+	267	WP_082785381.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G3T13_RS03325	691341..692204	-	864	WP_061774360.1	ROK family protein	-
G3T13_RS03330	692210..693634	-	1425	WP_061774361.1	glycoside hydrolase family 1 protein	-
G3T13_RS03335	693849..694544	-	696	WP_061774362.1	LrgB family protein	-
G3T13_RS03340	694541..694903	-	363	WP_061774363.1	CidA/LrgA family protein	-
G3T13_RS03345	695031..696242	-	1212	WP_082785383.1	CapA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-87)

Antitoxin

• Protein of unknown function DUF6290 (2-37)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10572.25 Da
• Isoelectric Point: 10.0256

>T149883 WP_082785381.1 NZ_CP048686:691006-691272 [Lactococcus raffinolactis]
VTYDFIPSPKFNRQFRILDKFTQKCIQNYINNVILKADNPHNHGKQLVGDLAVLWRYRIGDYRLIVTIQDNELMVLALQI
NHRRDIYR

Nucleotide

• Length: 267 bp

>T149883 NZ_CP064328:1307104-1307385 [Xylella fastidiosa subsp. fastidiosa]
ATGCGGAGGATTAGCCAGACTGGCCAATTCAAGCGCGACTACAAGCGCGAAGCTAAGGGCCAGCATCGCGCCACGCTTGA
TGAGGAACTGATACACGTCTTGGAAGCCTTGACCTGTGATCATCCGTTGGAGCCACGCCACCACGATCACGCACTAACTG
GCGACTGGAAAGATCACCGTGATTGCCACATCAAACCCGACTTGGTACTGATATACCGGAAGCCCGACAACGAGACATTG
CAGCTCGTGCGCATTGGCTCACACAGCGAACTAGGTTTGTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7328.24 Da
• Isoelectric Point: 4.1683

>AT149883 WP_061774359.1 NZ_CP048686:690825-691016 [Lactococcus raffinolactis]
MAKFEGLSLSESIKRTMVEKYENDYDLKVAETSIAEFQKYIISDKPVQSWEDLQPELGLDRDV

Nucleotide

• Length: 192 bp

>AT149883 NZ_CP064328:1306854-1307117 [Xylella fastidiosa subsp. fastidiosa]
ATGTCTGCAAACGCAGTTGTCCGTGCCCGCGTTGACGCGCACATCAAAGAAGAAGCATCAACCGTGCTGGCGACGATGGG
CCTAAGCGTGTCTGATGCGTTCCGTATGATGCTGACCCGCATTGCCCGCGAGAAGGCGCTGCCGTTCGAGCCGCTGGTGC
CGAACGAAACTACCATCGCAGCCATGCGTGAAGCCCGTGCTGGTCACCTCAAGAGCTTTGATAGTATTGAGGCGCTCATG
GCGGACTTGCATGCGGAGGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A2A5SDT8