Detailed information of TA system TA149878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2630..3214
• Replicon: plasmid pYH12138-7
• Accession: NZ_CP048677
• Organism: Acinetobacter sp. YH12138 strain YH12138_T

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: G0029_RS18035
• Protein ID: WP_000286964.1
• Coordinates: 2630..2932 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: G0029_RS18040
• Protein ID: WP_000985610.1
• Coordinates: 2933..3214 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0029_RS18010	1..933	+	933	WP_180108337.1	replication initiation protein RepM	-
G0029_RS18015	975..1541	+	567	WP_194087891.1	plasmid replication DNA-binding protein	-
G0029_RS18020	1562..1705	+	144	WP_180108338.1	hypothetical protein	-
G0029_RS18025	1901..2242	+	342	WP_104472901.1	hypothetical protein	-
G0029_RS18030	2311..2544	+	234	WP_111885980.1	hypothetical protein	-
G0029_RS18035	2630..2932	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G0029_RS18040	2933..3214	+	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
G0029_RS18045	3321..3587	-	267	WP_180110335.1	hypothetical protein	-
G0029_RS18050	3784..4116	+	333	WP_180110336.1	hypothetical protein	-
G0029_RS18055	4637..4951	+	315	WP_180036197.1	hypothetical protein	-
G0029_RS18060	5342..5656	+	315	WP_180108336.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..6280	6280

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T149878 WP_000286964.1 NZ_CP048677:2630-2932 [Acinetobacter sp. YH12138]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T149878 NZ_CP064326:c2476328-2475900 [Xylella fastidiosa subsp. multiplex]
ATGATTGTCTTGGATACCAATGTTGTTTCCGAAGCAATGAAACCCGAGCCGGATGCAGCAGTCCGAACCTGGCTCAACGA
GCAGATGAGCGTCACGTTGTACCTATCCAGTGTGACGTTATCTGAACTGTTGTTTGGAATCGCAGTGTTGCCCACAAGTA
AGCGCAAGGACATGTTGGCTCGTACCCTTGATGGCTTGCTTGATCTGTTCAATGAACGGGTGTTGCCGTTCGATACGGAC
GCGGCACGCCACTATGCTGAACTGGCAGTGAAAGCGAGAAACAATGGGCGCGGCTTTCCCACACCGGATGGCTATATCGC
GGCAATTGCTGCATCACGAGGTTACATAGTAGCTTCCCGCGACACCTCAGCCTATGAGTCAAGTGGTGTGCAAGTCATCA
ATCCGTGGCAATACAGCAAACAAACATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT149878 WP_000985610.1 NZ_CP048677:2933-3214 [Acinetobacter sp. YH12138]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT149878 NZ_CP064326:c2476583-2476329 [Xylella fastidiosa subsp. multiplex]
ATGGCCATGTTGACAGTGCGTAATTTGCCTGATGAAGTCCATCGCGCCTTGCGGGTTCGAGCCGCCACACACGGCCGCAG
TACAGAAGCGGAAGTACGTGAGATTTTAACGAACATAGTCAAGCCGGATGAGCGTGTTCGCATTGGCGATGCCTTGGCTA
TCCTGGGACGCGAGATCGGACTAAATAACGAGGATTTCACGACCTTTGATAAGGTGCGAGACAAGACGCAGGCCGAGCCG
ATGAGATTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94