Detailed information of TA system TA149876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 54678..56013
• Replicon: plasmid pYH12138-2
• Accession: NZ_CP048672
• Organism: Acinetobacter sp. YH12138 strain YH12138_T

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: G0029_RS17450
• Protein ID: WP_120376155.1
• Coordinates: 55066..56013 (+)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: G0029_RS17445
• Protein ID: WP_120376154.1
• Coordinates: 54678..55073 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0029_RS17425	51524..52228	+	705	WP_001067856.1	IS6-like element IS26 family transposase	-
G0029_RS17430	52320..52499	+	180	WP_000902128.1	hypothetical protein	-
G0029_RS17435	52653..53468	-	816	WP_000018323.1	APH(3')-I family aminoglycoside O-phosphotransferase	-
G0029_RS17440	53598..54302	+	705	WP_001067856.1	IS6-like element IS26 family transposase	-
G0029_RS17445	54678..55073	+	396	WP_120376154.1	S-adenosylhomocysteine hydrolase	Antitoxin
G0029_RS17450	55066..56013	+	948	WP_120376155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
G0029_RS17455	56211..56915	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
G0029_RS17460	56969..57403	+	435	Protein_53	recombinase family protein	-
G0029_RS17465	57655..58473	+	819	WP_032073151.1	AraC family transcriptional regulator	-
G0029_RS17470	58501..59097	-	597	WP_074163998.1	LysE family transporter	-
G0029_RS17475	59623..60687	+	1065	WP_169538922.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id / tet(Y) / aph(3')-Ia / sul2	-	1..105038	105038
	-	inside	IScluster/Tn	aph(3'')-Ib / aph(6)-Id / tet(Y) / aph(3')-Ia	-	43996..56915	12919

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-299)

Antitoxin

• Protein of unknown function DUF6088 (10-129)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 36241.70 Da
• Isoelectric Point: 6.3975

>T149876 WP_120376155.1 NZ_CP048672:55066-56013 [Acinetobacter sp. YH12138]
MPDIKNVFADVADALGIGATSIVEKDYYVVELLRLLQSLEFETHDLVFAGGTSLAKANIQLNRMSEDVDIKIVPKPNIAL
SRSKAKVVRKQLLHQVQDKISQSDFFTIEEEPVVWDEYRYMELLIRYPQHFNQAPCLRPFIKLELVETDLLEPFESRSIT
SFVYDLAKKGKPVDAFPSVTIVSTSIEKLIAILRRTAAFARNAERADDESLVRHVYDYFKITYGKPQDFVYLGHLATKVI
KTDIERYGRQHTLMVEDPIAELLFGLNELSRNPLYKARFNNFVVPMVFGDKQISWQQAYSHFQDSVQQIFANLKL

Nucleotide

• Length: 948 bp

>T149876 NZ_CP064326:1365321-1365587 [Xylella fastidiosa subsp. multiplex]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCACGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAATCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGTGGCT
TTTGGCGTTATCGTGTTGGTGATTTTCGGGTCGTCTGCGACATTCAAGATAGCGTTTTGCGCGTCCTTGTAGTGCGTGTT
GGTCACCGGGGCGAGATTTACCGATAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14884.31 Da
• Isoelectric Point: 10.6946

>AT149876 WP_120376154.1 NZ_CP048672:54678-55073 [Acinetobacter sp. YH12138]
MTTKAKMQMRIKRSKRYVFMRDDFNDIANYDQIGRVLRNLVQEGELIKVGYGIYTKARKNQITGKVMPACPEGADAVILE
ALKRLDVQVTLGEATEKYLSGQSTQIPAFTQIKTPRRFTRQLSVGRSQINA

Nucleotide

• Length: 396 bp

>AT149876 NZ_CP064326:1365110-1365337 [Xylella fastidiosa subsp. multiplex]
ATGGCGACTTCGATTCGCCTCTCTCCTGAAATGGAGCAAAGGCTTAACTCTCTTGCCTCCCATACGGGACGCACAAAGGC
TTACTACCTGCGTGAAATCATTGAGCATGGCATTGAGGAAATGGAGGATTACTACCTTGCTGCCGATGTGTTGGAGCGTG
TCCGTCATGGACAAGAACAGGTGCATTCTGCTGCCGACGTGAGGAAAACGCTTGGCCTGGACGATTGA