Detailed information of TA system TA149875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 104364..104952
• Replicon: plasmid pYH12138-1
• Accession: NZ_CP048671
• Organism: Acinetobacter sp. YH12138 strain YH12138_T

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: G0029_RS16620
• Protein ID: WP_092818268.1
• Coordinates: 104665..104952 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A1E8DZR6
• Locus tag: G0029_RS16615
• Protein ID: WP_070155399.1
• Coordinates: 104364..104678 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0029_RS16575	99575..100048	+	474	WP_180109979.1	hypothetical protein	-
G0029_RS16580	100186..101445	-	1260	WP_180108329.1	type II toxin-antitoxin system HipA family toxin	-
G0029_RS16585	101450..101752	-	303	WP_111886029.1	helix-turn-helix transcriptional regulator	-
G0029_RS16590	102071..102331	-	261	WP_180036186.1	helix-turn-helix domain-containing protein	-
G0029_RS16595	102749..103225	-	477	WP_180109926.1	hypothetical protein	-
G0029_RS16600	103311..103577	-	267	WP_180109927.1	hypothetical protein	-
G0029_RS16605	103764..103907	+	144	WP_180109928.1	toxin C-terminal domain-containing protein	-
G0029_RS16610	103913..104308	+	396	WP_180109929.1	hypothetical protein	-
G0029_RS16615	104364..104678	-	315	WP_070155399.1	BrnA antitoxin family protein	Antitoxin
G0029_RS16620	104665..104952	-	288	WP_092818268.1	BrnT family toxin	Toxin
G0029_RS16625	105182..105685	-	504	WP_016803256.1	hypothetical protein	-
G0029_RS16630	105687..106310	-	624	WP_180109930.1	hypothetical protein	-
G0029_RS16635	106316..106867	-	552	WP_180109931.1	PAAR domain-containing protein	-
G0029_RS16640	106860..107309	-	450	WP_078426712.1	PAAR domain-containing protein	-
G0029_RS16645	107534..107995	+	462	Protein_104	helix-turn-helix domain-containing protein	-
G0029_RS16650	107988..108137	-	150	Protein_105	IS5/IS1182 family transposase	-
G0029_RS16655	108835..109278	-	444	WP_180109932.1	hypothetical protein	-
G0029_RS16660	109278..109544	-	267	WP_180109980.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..207449	207449
	-	flank	IS/Tn	-	-	107534..108037	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.76 Da
• Isoelectric Point: 6.4846

>T149875 WP_092818268.1 NZ_CP048671:c104952-104665 [Acinetobacter sp. YH12138]
MKQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T149875 NZ_CP064326:1361748-1362041 [Xylella fastidiosa subsp. multiplex]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACGACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12199.87 Da
• Isoelectric Point: 8.4628

>AT149875 WP_070155399.1 NZ_CP048671:c104678-104364 [Acinetobacter sp. YH12138]
MSMVRYSRKELNEKFSEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRPNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT149875 NZ_CP064326:1361449-1361745 [Xylella fastidiosa subsp. multiplex]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1E8DZR6