Detailed information of TA system TA149868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 3366..3943
• Replicon: plasmid pYH12207-4
• Accession: NZ_CP048663
• Organism: Acinetobacter piscicola strain YH12207_T

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: N8ZPI0
• Locus tag: G0028_RS20635
• Protein ID: WP_004812233.1
• Coordinates: 3366..3614 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: N8ZW11
• Locus tag: G0028_RS20640
• Protein ID: WP_004812232.1
• Coordinates: 3611..3943 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0028_RS20625	1413..2531	-	1119	WP_180047661.1	Fic family protein	-
G0028_RS20630	2733..3260	+	528	WP_180047663.1	plasmid pRiA4b ORF-3 family protein	-
G0028_RS20635	3366..3614	+	249	WP_004812233.1	type II toxin-antitoxin system HicA family toxin	Toxin
G0028_RS20640	3611..3943	+	333	WP_004812232.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
G0028_RS20645	4073..4408	+	336	WP_179997550.1	hypothetical protein	-
G0028_RS20650	4458..4637	+	180	WP_001231652.1	hypothetical protein	-
G0028_RS20655	4634..5020	+	387	WP_000053135.1	type II toxin-antitoxin system death-on-curing family toxin	-
G0028_RS20660	5022..5573	-	552	WP_180042379.1	macro domain-containing protein	-
G0028_RS20665	5582..6280	-	699	WP_180047665.1	hypothetical protein	-
G0028_RS20670	6634..6858	-	225	WP_004758944.1	hypothetical protein	-
G0028_RS20675	7012..7257	+	246	WP_004282220.1	hypothetical protein	-
G0028_RS20680	7547..8308	-	762	WP_057073610.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..13003	13003

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-78)

Antitoxin

• Uncharacterised protein family HicB (54-101)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9436.02 Da
• Isoelectric Point: 10.1337

>T149868 WP_004812233.1 NZ_CP048663:3366-3614 [Acinetobacter piscicola]
MGKTEKLLEKFGNSKNTFPYKDLVVLLSQLGYEKFEMAGSRVRFFNEKTEHMILLHRPHPENEIKGGALKAVKQALKQEG
FL

Nucleotide

• Length: 249 bp

>T149868 NZ_CP064325:1359514-1359780 [Xylella fastidiosa subsp. multiplex]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAGCAGCAGTTACGCAAGCTCGACAAGCACATGGCACGGCGCATTGT
CGATTTCATGGATGAGCGCATCGCCGGACTAGAAAATCCACGCAGCAGTGGTAAAGCATTAACCGGACCACTTGGTGGCT
TTTGGCGTTATCGTGTTGGTGATTTTCGGGTCGTCTGCGACATTCAAGATAGCGTTTTGCGCGTCCTTGTAGTGCGTGTT
GGTCACCGGGGCGAGATTTACCGATAG

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12224.97 Da
• Isoelectric Point: 4.8951

>AT149868 WP_004812232.1 NZ_CP048663:3611-3943 [Acinetobacter piscicola]
MSYLKYKGYLGTIEPDLETGELFGKLAFIRDLITYEAETLKALEKAFQESVDGYLESCAELGKSPDQPFKGTFNVRISPE
LHRKAVLASSNSLNAFVSDAIQEKLMRLGA

Nucleotide

• Length: 333 bp

>AT149868 NZ_CP064325:1359303-1359530 [Xylella fastidiosa subsp. multiplex]
ATGGCGACTTCGATTCGCCTCTCTCCTGAAATGGAGCAAAGGCTTAACTCTCTTGCCTCCCATACGGGACGCACAAAGGC
TTACTACCTGCGTGAAATCATTGAGCATGGCATTGAGGAAATGGAGGATTACTACCTTGCTGCCGATGTGTTGGAGCGTG
TCCGTCATGGACAAGAACAGGTGCATTCTGCTGCCGACGTGAGGAAAACGCTTGGCCTGGACGATTGA

Structures

Toxin

• AlphaFold DB: N8ZPI0

Antitoxin

• AlphaFold DB: N8ZW11