Detailed information of TA system TA149864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 12365..12953
• Replicon: plasmid pC15-3
• Accession: NZ_CP048657
• Organism: Acinetobacter indicus strain C15_T

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0A3W8D4
• Locus tag: G0027_RS16435
• Protein ID: WP_034583450.1
• Coordinates: 12657..12953 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: G0027_RS16430
• Protein ID: WP_000241337.1
• Coordinates: 12365..12655 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0027_RS16415	9465..10214	-	750	WP_163143588.1	fimbria/pilus periplasmic chaperone	-
G0027_RS16420	10258..10737	-	480	WP_180192429.1	spore coat protein U domain-containing protein	-
G0027_RS16425	11001..12220	+	1220	WP_194088155.1	IS3 family transposase	-
G0027_RS16430	12365..12655	-	291	WP_000241337.1	putative addiction module antidote protein	Antitoxin
G0027_RS16435	12657..12953	-	297	WP_034583450.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G0027_RS16440	13284..14609	-	1326	WP_179994539.1	ISNCY family transposase	-
G0027_RS16445	14845..16707	-	1863	WP_138743310.1	AAA family ATPase	-
G0027_RS16450	16924..17067	-	144	WP_166135762.1	hypothetical protein	-
G0027_RS16455	17087..17662	-	576	WP_180192427.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..31061	31061
	-	inside	IScluster/Tn	-	-	11001..14621	3620

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11583.27 Da
• Isoelectric Point: 7.0148

>T149864 WP_034583450.1 NZ_CP048657:c12953-12657 [Acinetobacter indicus]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDINRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T149864 NZ_CP064325:c1026436-1025951 [Xylella fastidiosa subsp. multiplex]
GTGAATATGCAGATTTCGACACCTCATTCACTCACGGCAGCGCATCGACTTGATGAATTCAATTGCGGTGAGCCATCGCT
GGATGATTGGCTTAAACGACGCGCACTGACCAACCATTTAAACGGGGCCAGCCGGACCTTCGTGGTTATCGATGCCAACC
AATACGTGCTTGGGTACTACGCTTTGGCAGCCGGGGCTGTAGCGCATCAAGAGGCCACCCGTGCTATCCGACGTAACATG
CCTGATCCAGTGCCGGTCATGGTGCTCGGAAGGCTGGCTGTGGATACTCGTGCTCAAGGCATCAAACTCGGTGCGGCCTT
GCTTAAAGATACGGTAACTCGTGTGCAGTCCATTGCAGAGAACGCCGGCGTCAGGGCATTGGTAGTCCATGCGTTGAACG
AGCGCGCAAAGCAATTCTACGAGTACTATGGTTTTCGAGCGTCACCAATGCACCTGATGACCTTGATGCTTCCGATTAAA
TTCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10584.24 Da
• Isoelectric Point: 5.1133

>AT149864 WP_000241337.1 NZ_CP048657:c12655-12365 [Acinetobacter indicus]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSTPRFDTI
NRVVNALGLKLTVQHT

Nucleotide

• Length: 291 bp

>AT149864 NZ_CP064325:c1026705-1026433 [Xylella fastidiosa subsp. multiplex]
ATGCGCGACGCTTCCATCAACTTACGTGCCATGCCTGAGCAGCGCGACTTGATTGACCAGGCTGCTAATCTATTAGGCAA
GAATCGCTCAGACTTCATGCTAGAAGCTGCGTGCGAGCGTGCGAAAGCAATTATCCTGGATCAAGTCTTTTTCAATCTGG
ACGAAGAGAGGTTCCGGCAATTCACTGTGTTGCTTAATGCGCCACAAGACACCAATCCAGGCCTTAATCGCCTGATGGCA
ATTAAGGCTCCGTGGAGCATCAGCAATACGTGA

Structures

Toxin

• AlphaFold DB: A0A0A3W8D4