Detailed information of TA system TA149862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 62550..63885
• Replicon: plasmid pC15-1
• Accession: NZ_CP048655
• Organism: Acinetobacter indicus strain C15_T

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: G0027_RS15875
• Protein ID: WP_120376155.1
• Coordinates: 62938..63885 (+)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: G0027_RS15870
• Protein ID: WP_120376154.1
• Coordinates: 62550..62945 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0027_RS15850	58358..59490	-	1133	Protein_58	IS3-like element ISAba14 family transposase	-
G0027_RS15855	59509..60648	+	1140	Protein_59	ISNCY family transposase	-
G0027_RS15860	60691..61395	+	705	WP_005028420.1	IS6 family transposase	-
G0027_RS15865	61470..62174	+	705	WP_001067856.1	IS6-like element IS26 family transposase	-
G0027_RS15870	62550..62945	+	396	WP_120376154.1	S-adenosylhomocysteine hydrolase	Antitoxin
G0027_RS15875	62938..63885	+	948	WP_120376155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
G0027_RS15880	64083..64787	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
G0027_RS15885	64841..65275	+	435	Protein_65	recombinase family protein	-
G0027_RS15890	65527..66345	+	819	WP_032073151.1	AraC family transcriptional regulator	-
G0027_RS15895	66373..66969	-	597	WP_074163998.1	LysE family transporter	-
G0027_RS15900	67495..68559	+	1065	WP_169538922.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / blaOXA-58 / aac(3)-IId / dfrA16 / blaCARB-2 / aadA2	-	1..104003	104003
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	46070..64787	18717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-299)

Antitoxin

• Protein of unknown function DUF6088 (10-129)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 36241.70 Da
• Isoelectric Point: 6.3975

>T149862 WP_120376155.1 NZ_CP048655:62938-63885 [Acinetobacter indicus]
MPDIKNVFADVADALGIGATSIVEKDYYVVELLRLLQSLEFETHDLVFAGGTSLAKANIQLNRMSEDVDIKIVPKPNIAL
SRSKAKVVRKQLLHQVQDKISQSDFFTIEEEPVVWDEYRYMELLIRYPQHFNQAPCLRPFIKLELVETDLLEPFESRSIT
SFVYDLAKKGKPVDAFPSVTIVSTSIEKLIAILRRTAAFARNAERADDESLVRHVYDYFKITYGKPQDFVYLGHLATKVI
KTDIERYGRQHTLMVEDPIAELLFGLNELSRNPLYKARFNNFVVPMVFGDKQISWQQAYSHFQDSVQQIFANLKL

Nucleotide

• Length: 948 bp

>T149862 NZ_CP064325:460330-460611 [Xylella fastidiosa subsp. multiplex]
ATGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTCTTCCTTGTGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCCAAGCGCCCCGAAGATATGAATGCTCCAGGCTGGAAACTGC
ACCCGCTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTGAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTACCACTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14884.31 Da
• Isoelectric Point: 10.6946

>AT149862 WP_120376154.1 NZ_CP048655:62550-62945 [Acinetobacter indicus]
MTTKAKMQMRIKRSKRYVFMRDDFNDIANYDQIGRVLRNLVQEGELIKVGYGIYTKARKNQITGKVMPACPEGADAVILE
ALKRLDVQVTLGEATEKYLSGQSTQIPAFTQIKTPRRFTRQLSVGRSQINA

Nucleotide

• Length: 396 bp

>AT149862 NZ_CP064325:460622-460897 [Xylella fastidiosa subsp. multiplex]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGATCTCACTGTGACCAATGCGGC
TGCAAAGCTAGGCGTCAGCCGTGTCGCTTTGTCGCGTATTCTCAATGGTACCAATGGCATCTCTGCCGATATGGCCCTTC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGCAGCCATGCAGGCCAAGTATGACCTGTGGGTTGCTAGCCAG
CAGCCACGCCCGAAGATCACACGTCTTCATGTGTAA