Detailed information of TA system TA149860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 48806..49390
• Replicon: plasmid pC15-1
• Accession: NZ_CP048655
• Organism: Acinetobacter indicus strain C15_T

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: G0027_RS15800
• Protein ID: WP_000286964.1
• Coordinates: 48806..49108 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: G0027_RS15805
• Protein ID: WP_000985609.1
• Coordinates: 49109..49390 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0027_RS15770	44139..44870	+	732	WP_005245162.1	transglutaminase family protein	-
G0027_RS15775	44857..45744	+	888	WP_127800877.1	cation diffusion facilitator family transporter	-
G0027_RS15780	45829..45987	-	159	Protein_44	efflux RND transporter permease subunit	-
G0027_RS15785	46070..46498	+	429	WP_194088148.1	transposase	-
G0027_RS15790	46495..46830	+	336	WP_004783682.1	IS66 family insertion sequence element accessory protein TnpB	-
G0027_RS15795	46905..48614	+	1710	WP_180192466.1	IS66 family transposase	-
G0027_RS15800	48806..49108	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G0027_RS15805	49109..49390	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
G0027_RS15810	49481..50806	-	1326	WP_179994539.1	ISNCY family transposase	-
G0027_RS15815	50957..51376	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
G0027_RS15820	51555..52880	+	1326	WP_179994539.1	ISNCY family transposase	-
G0027_RS15825	53004..53324	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
G0027_RS15830	53317..53589	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / blaOXA-58 / aac(3)-IId / dfrA16 / blaCARB-2 / aadA2	-	1..104003	104003
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	46070..64787	18717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T149860 WP_000286964.1 NZ_CP048655:48806-49108 [Acinetobacter indicus]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T149860 NZ_CP064316:c2678406-2678023 [Lacticaseibacillus paracasei subsp. tolerans]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCAGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGCTTGGTTAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT149860 WP_000985609.1 NZ_CP048655:49109-49390 [Acinetobacter indicus]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT149860 NZ_CP064316:c2678674-2678426 [Lacticaseibacillus paracasei subsp. tolerans]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCGGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94