Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 49413..50056 Replicon plasmid unnamed1
Accession NZ_CP048651
Organism Enterobacter roggenkampii strain Ecl_20_981

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag G3T53_RS24665 Protein ID WP_000754567.1
Coordinates 49413..49829 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag G3T53_RS24670 Protein ID WP_023293777.1
Coordinates 49826..50056 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3T53_RS24635 44599..45180 + 582 WP_008502230.1 TetR/AcrR family transcriptional regulator -
G3T53_RS24640 45185..45523 + 339 WP_000888080.1 carboxymuconolactone decarboxylase family protein -
G3T53_RS24645 45553..45882 - 330 WP_008502229.1 thioredoxin family protein -
G3T53_RS24650 46095..47201 + 1107 WP_006687059.1 alkene reductase -
G3T53_RS24655 47267..47968 + 702 WP_008502228.1 DsbA family oxidoreductase -
G3T53_RS24660 48094..49062 + 969 WP_084832609.1 IS5 family transposase -
G3T53_RS24665 49413..49829 - 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
G3T53_RS24670 49826..50056 - 231 WP_023293777.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G3T53_RS24675 50194..50676 + 483 WP_188074410.1 hypothetical protein -
G3T53_RS24680 50965..52170 + 1206 WP_006797591.1 AAA family ATPase -
G3T53_RS24685 52170..53144 + 975 WP_000064119.1 ParB/RepB/Spo0J family partition protein -
G3T53_RS24690 53226..54497 - 1272 WP_032627083.1 Y-family DNA polymerase -
G3T53_RS24695 54497..54928 - 432 WP_006796638.1 translesion error-prone DNA polymerase V autoproteolytic subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-10 - 1..161986 161986


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T149856 WP_000754567.1 NZ_CP048651:c49829-49413 [Enterobacter roggenkampii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T149856 NZ_CP064311:c2761904-2761521 [Lacticaseibacillus paracasei subsp. tolerans]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTCGAACGCAATTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGATCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT149856 WP_023293777.1 NZ_CP048651:c50056-49826 [Enterobacter roggenkampii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT149856 NZ_CP064311:c2762172-2761924 [Lacticaseibacillus paracasei subsp. tolerans]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References