Detailed information of TA system TA149854

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4313413..4314092
• Replicon: chromosome
• Accession: NZ_CP048650
• Organism: Enterobacter roggenkampii strain Ecl_20_981

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A1Z3N0J5
• Locus tag: G3T53_RS20715
• Protein ID: WP_045268641.1
• Coordinates: 4313413..4313754 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A1Z3N0L6
• Locus tag: G3T53_RS20720
• Protein ID: WP_045268640.1
• Coordinates: 4313775..4314092 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3T53_RS20695	4308700..4310445	+	1746	WP_023294585.1	DNA primase	-
G3T53_RS20700	4310600..4312447	+	1848	WP_008503184.1	RNA polymerase sigma factor RpoD	-
G3T53_RS20705	4312545..4313051	-	507	WP_032675517.1	G/U mismatch-specific DNA glycosylase	-
G3T53_RS20715	4313413..4313754	-	342	WP_045268641.1	TA system toxin CbtA family protein	Toxin
G3T53_RS20720	4313775..4314092	-	318	WP_045268640.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G3T53_RS20725	4314112..4314333	-	222	WP_106929599.1	DUF987 domain-containing protein	-
G3T53_RS20730	4314342..4314818	-	477	WP_023336794.1	RadC family protein	-
G3T53_RS20735	4314834..4315292	-	459	WP_000211838.1	antirestriction protein	-
G3T53_RS20740	4315390..4315629	-	240	WP_000194654.1	DUF905 domain-containing protein	-
G3T53_RS20745	4315706..4316173	-	468	WP_001581459.1	hypothetical protein	-
G3T53_RS20750	4316197..4316640	-	444	WP_000824223.1	hypothetical protein	-
G3T53_RS20755	4316753..4317160	+	408	WP_016946740.1	transposase	-
G3T53_RS20760	4317157..4317507	+	351	WP_044596438.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4312545..4323501	10956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13045.30 Da
• Isoelectric Point: 10.5786

>T149854 WP_045268641.1 NZ_CP048650:c4313754-4313413 [Enterobacter roggenkampii]
MKTLPATTLRVVKPCLSPVAIWKMLLTRLLEQHYGLTLNDTPFSKERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRKNLVR

Nucleotide

• Length: 342 bp

>T149854 NZ_CP064311:c511620-511252 [Lacticaseibacillus paracasei subsp. tolerans]
ATGTATAAAATTGACTTCTATGAGGATCGCGATGGCTACTCTGAGATCGAAGACTTTCTTGATCAGTTACGCCATAGTCA
TCAAAAAAATAATGTGAGCCTTTTAAACAAAATCACAAAGGAACTCTACATGCTGCAAAATCTTGGTCCGCGCTTACGTG
AACCTCACGCGAAGTTTCTGAAGGGATATGCTTATCCGATCATGGAATTGCGTCCCATGCCAGAAAGAATCTTTTATGCT
GCATGGCAAAAGGATCATTTCGTTTTGCTTCATCACTACACCAAGAAAACAAACAAAACTGATCACAGAGAAATTTTACG
GGCACAGGCTAACCTCGAAGATTGGCTCTCTAGAAAGGAAGAAAATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11976.67 Da
• Isoelectric Point: 6.7379

>AT149854 WP_045268640.1 NZ_CP048650:c4314092-4313775 [Enterobacter roggenkampii]
MTHIIWGLQRNITPRLGARLVQEGNRLHYLADRASITGMFSDVECLKLDDTFQHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT149854 NZ_CP064311:c511252-510992 [Lacticaseibacillus paracasei subsp. tolerans]
ATGACGACATGGGAAGACTTCAGTCAGAAACTGAAGAAGGACAATCCAGAAAAGTTGGCAATCATCAAAACCTTGGCCGA
TTTAGAAAGTGAACGAATCAAGCAAGGAATCTCTCAAAAGGACTTTGCAGAAAAAATTGGTATGAAACAGCCGCAACTAG
CAAAGATTGAGCGCTTAGACTCTCTTCCGACTCTGGCAACACTCAATCGATACGCGAGAGGGCTTGGAAAAGAAATTCGT
TTATCAATTGTTACTCAGTGA

Structures

Toxin

• AlphaFold DB: A0A1Z3N0J5

Antitoxin

• AlphaFold DB: A0A1Z3N0L6