Detailed information of TA system TA149853

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4206081..4206834
• Replicon: chromosome
• Accession: NZ_CP048650
• Organism: Enterobacter roggenkampii strain Ecl_20_981

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: G3T53_RS20165
• Protein ID: WP_016154457.1
• Coordinates: 4206457..4206834 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: G3T53_RS20160
• Protein ID: WP_162838704.1
• Coordinates: 4206081..4206407 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3T53_RS20100	4201139..4201405	+	267	WP_009652486.1	hypothetical protein	-
G3T53_RS20105	4201418..4201729	+	312	WP_009652410.1	hypothetical protein	-
G3T53_RS20110	4201776..4202246	+	471	WP_009652431.1	hypothetical protein	-
G3T53_RS20115	4202295..4202807	+	513	WP_009652395.1	DUF4234 domain-containing protein	-
G3T53_RS20120	4202890..4203111	+	222	WP_009652443.1	hypothetical protein	-
G3T53_RS20125	4203167..4203400	+	234	WP_009652474.1	DUF905 domain-containing protein	-
G3T53_RS20130	4203504..4204322	+	819	WP_009652424.1	DUF945 domain-containing protein	-
G3T53_RS20135	4204322..4204576	+	255	WP_009652519.1	hypothetical protein	-
G3T53_RS20140	4204605..4205066	+	462	WP_045890390.1	antirestriction protein	-
G3T53_RS20145	4205077..4205295	+	219	WP_009652503.1	hypothetical protein	-
G3T53_RS20150	4205307..4205789	+	483	WP_016154455.1	DNA repair protein RadC	-
G3T53_RS20155	4205803..4206024	+	222	WP_009652392.1	DUF987 domain-containing protein	-
G3T53_RS20160	4206081..4206407	+	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G3T53_RS20165	4206457..4206834	+	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
G3T53_RS20170	4206831..4207322	+	492	WP_009652438.1	hypothetical protein	-
G3T53_RS20175	4207352..4207555	+	204	WP_009652505.1	DUF957 domain-containing protein	-
G3T53_RS20180	4207636..4208481	+	846	Protein_3950	DUF4942 domain-containing protein	-
G3T53_RS20185	4208493..4208849	+	357	WP_188074296.1	hypothetical protein	-
G3T53_RS20190	4209436..4210023	+	588	WP_049017571.1	hypothetical protein	-
G3T53_RS20195	4210370..4211557	+	1188	WP_042111462.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T149853 WP_016154457.1 NZ_CP048650:4206457-4206834 [Enterobacter roggenkampii]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T149853 NZ_CP064307:c34085-33804 [Lacticaseibacillus paracasei subsp. tolerans]
ATGAATAAGTTTATTTATAAACCAGCCTTTGAACGTCAATTTAAAAAACATTACAAGACGATTTTAAAAGGGGGGCGCTA
TAAAAAAGAAGATTTTGAAGCTGTTTATCATAAACTATTACAAGATGAACCGTTAGATCCACATTATAATGATCACGCTT
TGGTTAATCGTAAACCAGAGCGTGATTTACATATCAAGCCGGATTGGTTATTAATTTACAAATATGATGGTGAGTTTGTG
CGCTTTATCGACACGGGTTCGCATTCAGATCTTTTTAATTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT149853 WP_162838704.1 NZ_CP048650:4206081-4206407 [Enterobacter roggenkampii]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT149853 NZ_CP064307:c34320-34078 [Lacticaseibacillus paracasei subsp. tolerans]
ATGACTAGCCAAGTACATTTCAGAATGAGCGAAAAAGAAAAAAAGGACTTCGAGATTGTCCTAAGTCGTGTGGGATTAAC
TCCCGGAGAAGCCTTTCGTATCTTTGCCAAGAAATCCATTGAAGCCGGAGGAATTCCCTTTGAGGTCTCTCAGCCAAACA
CACGTTTAAAAAAGGCTATTAGAAGTCAAGATTACGTTGAATTTAACGATCCTGAGGAAGGGCTGGATTGGTTAAATGAA
TAA

Structures

Toxin

• AlphaFold DB: R8WN26