Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 3531649..3532313 Replicon chromosome
Accession NZ_CP048650
Organism Enterobacter roggenkampii strain Ecl_20_981

Toxin (Protein)


Gene name ykfI Uniprot ID A0A157QTP3
Locus tag G3T53_RS16930 Protein ID WP_007869731.1
Coordinates 3531987..3532313 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0M7FI99
Locus tag G3T53_RS16925 Protein ID WP_007869729.1
Coordinates 3531649..3531966 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3T53_RS16885 3527728..3528465 + 738 WP_013098169.1 WYL domain-containing protein -
G3T53_RS16890 3528522..3528794 + 273 WP_007869719.1 hypothetical protein -
G3T53_RS16895 3528874..3529134 + 261 WP_007869721.1 hypothetical protein -
G3T53_RS16900 3529261..3530082 + 822 WP_111986661.1 DUF945 domain-containing protein -
G3T53_RS16905 3530106..3530345 + 240 WP_007869725.1 DUF905 domain-containing protein -
G3T53_RS16910 3530449..3530907 + 459 WP_013098170.1 antirestriction protein -
G3T53_RS16915 3530923..3531399 + 477 WP_013098171.1 RadC family protein -
G3T53_RS16920 3531408..3531635 + 228 WP_013098172.1 DUF987 domain-containing protein -
G3T53_RS16925 3531649..3531966 + 318 WP_007869729.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
G3T53_RS16930 3531987..3532313 + 327 WP_007869731.1 TA system toxin CbtA family protein Toxin
G3T53_RS16935 3532541..3533872 - 1332 WP_044597861.1 type VI secretion system ImpA and VasL domain-containing protein -
G3T53_RS16940 3533974..3535566 - 1593 WP_044596437.1 IS66 family transposase -
G3T53_RS16945 3535597..3535947 - 351 WP_044596438.1 IS66 family insertion sequence element accessory protein TnpB -
G3T53_RS16950 3535944..3536351 - 408 WP_016946740.1 transposase -
G3T53_RS16955 3536479..3536949 - 471 WP_025912748.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - tssG / tssF / icmF/tssM 3504442..3552854 48412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-106)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12299.25 Da        Isoelectric Point: 8.9951

>T149851 WP_007869731.1 NZ_CP048650:3531987-3532313 [Enterobacter roggenkampii]
MKTLPATTQRAAKPCLSPVAVWQMLLARLLEQHYGLTINDTPFSDEVVIQQHIDAGITLSDAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQAIGLLRGSH

Download         Length: 327 bp

>T149851 NZ_CP064304:1569651-1570019 [Lacticaseibacillus paracasei subsp. tolerans]
ATGTATAAAATTGACTTCTATGAGGATCGCGATGGCTACTCTGAGATCGAAGACTTTCTTGATCAGTTACGCCATAGTCA
TCAAAAAAATAATGTGAGCCTTTTAAACAAAATCACAAAGGAACTCTACATGCTGCAAAATCTTGGTCCGCGCTTACGTG
AACCTCACGCGAAGTTTCTGAAGGGATATGCTTATCCGATCATGGAATTGCGTCCCATGCCAGAAAGAATCTTTTATGCT
GCATGGCAAAAGGATCATTTCGTTTTGCTTCATCACTACACCAAGAAAACAAACAAAACTGATCACAGAGAAATTTTACG
GGCACAGTCTAACCTCGAAGATTGGCTCTCTAGAAAGGAAGAAAATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11917.60 Da        Isoelectric Point: 7.0264

>AT149851 WP_007869729.1 NZ_CP048650:3531649-3531966 [Enterobacter roggenkampii]
MTHIIWGLQRAITPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT149851 NZ_CP064304:1570019-1570279 [Lacticaseibacillus paracasei subsp. tolerans]
ATGACGACATGGGAAGACTTCAGTCAGAAACTGAAGAAGGACAATCCAGAAAAGTTGGCAATCATCAAAACCTTGGCCGA
TTTAGAAAGTGAACGAATCAAGCAAGGAATCTCTCAAAAGGACTTTGCAGAAAAAATTGGTATGAAACAGCCGCAACTAG
CAAAGATTGAGCGCTTAGACTCTCTTCCGACTCTGGCAACACTCAATCGATACGCGAGAGGGCTTGGAAAAGAAATTCGT
TTATCAATTGTTACTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A157QTP3

Antitoxin

Source ID Structure
AlphaFold DB A0A0M7FI99

References