Detailed information of TA system TA149840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 45737..46398
• Replicon: chromosome
• Accession: NZ_CP048650
• Organism: Enterobacter roggenkampii strain Ecl_20_981

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G3T53_RS00235
• Protein ID: WP_023293716.1
• Coordinates: 46000..46398 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A156KC23
• Locus tag: G3T53_RS00230
• Protein ID: WP_032675702.1
• Coordinates: 45737..46003 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3T53_RS00205	41790..43118	-	1329	WP_025910681.1	PTS sugar transporter subunit IIC	-
G3T53_RS00210	43130..43444	-	315	WP_008500132.1	PTS sugar transporter subunit IIB	-
G3T53_RS00215	43738..44688	+	951	WP_023293714.1	LacI family transcriptional regulator	-
G3T53_RS00220	44750..45046	-	297	WP_044597403.1	hypothetical protein	-
G3T53_RS00225	45188..45607	+	420	WP_008500129.1	GNAT family N-acetyltransferase	-
G3T53_RS00230	45737..46003	+	267	WP_032675702.1	virulence protein	Antitoxin
G3T53_RS00235	46000..46398	+	399	WP_023293716.1	type II toxin-antitoxin system VapC family toxin	Toxin
G3T53_RS00240	46538..47338	+	801	WP_008500128.1	lipoprotein NlpA	-
G3T53_RS00245	47461..48240	+	780	WP_044597402.1	MBL fold metallo-hydrolase	-
G3T53_RS00250	48267..49220	+	954	WP_044597401.1	helix-turn-helix domain-containing protein	-
G3T53_RS00255	49460..51100	+	1641	WP_044598011.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14799.00 Da
• Isoelectric Point: 6.4779

>T149840 WP_023293716.1 NZ_CP048650:46000-46398 [Enterobacter roggenkampii]
MRHMLDTNIVSHLFKRHPEVVSRMTCLAPGDVCISSITEAELLYGADKKKSSRLKETIREFLNTITICDWDSDAAATYGE
LRATLEKKGKVMGDLDQLIAAHAISRGTTIVTNDRAFRMVQELAVEDWTTTL

Nucleotide

• Length: 399 bp

>T149840 NZ_CP064260:44412-44687 [Klebsiella pneumoniae]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10009.34 Da
• Isoelectric Point: 4.6310

>AT149840 WP_032675702.1 NZ_CP048650:45737-46003 [Enterobacter roggenkampii]
MERTAKLFRKGQNQAVMLPAEFAFDTESVYIRRDEEGNVVLAAKPVLIKSWDNFLRMLAETDVPDTFLSKEERNQSVTTR
DPLDGACS

Nucleotide

• Length: 267 bp

>AT149840 NZ_CP064260:44128-44412 [Klebsiella pneumoniae]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A156KC23