Detailed information of TA system TA149837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4234585..4235405
• Replicon: chromosome
• Accession: NZ_CP048647
• Organism: Escherichia coli strain GW-AmxH19

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: G3273_RS20270
• Protein ID: WP_001054376.1
• Coordinates: 4234585..4234842 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: G3273_RS20275
• Protein ID: WP_001519303.1
• Coordinates: 4234854..4235405 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3273_RS20250	4229871..4230977	+	1107	WP_001519308.1	N-acetylneuraminate epimerase	-
G3273_RS20255	4231042..4232022	+	981	WP_001519307.1	transposase	-
G3273_RS20260	4232605..4233846	-	1242	Protein_3975	DNA helicase	-
G3273_RS20265	4233909..4234208	+	300	WP_001519304.1	GNAT family N-acetyltransferase	-
G3273_RS20270	4234585..4234842	+	258	WP_001054376.1	hypothetical protein	Toxin
G3273_RS20275	4234854..4235405	+	552	WP_001519303.1	N-acetyltransferase	Antitoxin
G3273_RS24865	4235457..4235618	+	162	WP_001519302.1	hypothetical protein	-
G3273_RS20280	4235655..4236203	+	549	WP_001519301.1	hypothetical protein	-
G3273_RS20285	4236330..4236590	+	261	WP_000084876.1	hypothetical protein	-
G3273_RS24915	4236628..4236744	+	117	Protein_3982	VOC family protein	-
G3273_RS20290	4236989..4238110	+	1122	WP_000010833.1	M42 family metallopeptidase	-
G3273_RS20295	4238107..4238385	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
G3273_RS20300	4238397..4239710	+	1314	WP_001519300.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	4225995..4243625	17630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T149837 WP_001054376.1 NZ_CP048647:4234585-4234842 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T149837 NZ_CP064259:c376737-376432 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT149837 WP_001519303.1 NZ_CP048647:4234854-4235405 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT149837 NZ_CP064259:c376957-376739 [Klebsiella pneumoniae]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: S1PA82