Detailed information of TA system TA149834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4191739..4192247
• Replicon: chromosome
• Accession: NZ_CP048647
• Organism: Escherichia coli strain GW-AmxH19

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: G3273_RS20070
• Protein ID: WP_001534843.1
• Coordinates: 4192038..4192247 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: G3273_RS20065
• Protein ID: WP_000063161.1
• Coordinates: 4191739..4192041 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3273_RS20050	4188267..4190417	+	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-
G3273_RS20055	4190535..4190738	+	204	WP_001534846.1	YbdD/YjiX family protein	-
G3273_RS20060	4190749..4191705	+	957	WP_001534845.1	GTPase	-
G3273_RS20065	4191739..4192041	-	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
G3273_RS20070	4192038..4192247	-	210	WP_001534843.1	BrnT family toxin	Toxin
G3273_RS20075	4192343..4193509	+	1167	WP_001534841.1	restriction endonuclease	-
G3273_RS20080	4193573..4195191	+	1619	Protein_3939	type I restriction-modification system subunit M	-
G3273_RS20085	4195181..4196491	+	1311	WP_001534837.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T149834 WP_001534843.1 NZ_CP048647:c4192247-4192038 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T149834 NZ_CP064259:c296308-295997 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT149834 WP_000063161.1 NZ_CP048647:c4192041-4191739 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT149834 NZ_CP064259:c296000-295581 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3