Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3649025..3649643 Replicon chromosome
Accession NZ_CP048647
Organism Escherichia coli strain GW-AmxH19

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag G3273_RS17550 Protein ID WP_001291435.1
Coordinates 3649425..3649643 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag G3273_RS17545 Protein ID WP_000344800.1
Coordinates 3649025..3649399 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3273_RS17535 3644115..3645308 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
G3273_RS17540 3645331..3648480 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
G3273_RS17545 3649025..3649399 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
G3273_RS17550 3649425..3649643 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
G3273_RS17555 3649816..3650367 + 552 WP_000102539.1 maltose O-acetyltransferase -
G3273_RS17560 3650483..3650953 + 471 WP_000136192.1 YlaC family protein -
G3273_RS17565 3651117..3652667 + 1551 WP_001468081.1 cyclic-guanylate-specific phosphodiesterase PdeB -
G3273_RS17570 3652709..3653062 - 354 WP_000878142.1 DUF1428 family protein -
G3273_RS17580 3653441..3653752 + 312 WP_000409908.1 MGMT family protein -
G3273_RS17585 3653783..3654355 - 573 WP_000779844.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149830 WP_001291435.1 NZ_CP048647:3649425-3649643 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T149830 NZ_CP064258:c4905032-4904748 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT149830 WP_000344800.1 NZ_CP048647:3649025-3649399 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT149830 NZ_CP064258:c4905264-4905022 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References