Detailed information of TA system TA149802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1060347..1060876
• Replicon: chromosome
• Accession: NZ_CP048643
• Organism: Staphylococcus aureus strain SR153

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GAU34_RS05180
• Protein ID: WP_000621175.1
• Coordinates: 1060514..1060876 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: GAU34_RS05175
• Protein ID: WP_000948331.1
• Coordinates: 1060347..1060517 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAU34_RS05145	1055384..1055944	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
GAU34_RS05150	1056152..1056631	+	480	WP_001287087.1	hypothetical protein	-
GAU34_RS05155	1056624..1058207	+	1584	WP_001294626.1	PH domain-containing protein	-
GAU34_RS05160	1058194..1058685	+	492	WP_001205912.1	PH domain-containing protein	-
GAU34_RS05165	1058689..1059048	+	360	WP_000581197.1	holo-ACP synthase	-
GAU34_RS05170	1059114..1060262	+	1149	WP_001281154.1	alanine racemase	-
GAU34_RS05175	1060347..1060517	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GAU34_RS05180	1060514..1060876	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GAU34_RS05185	1061226..1062227	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
GAU34_RS05190	1062346..1062672	+	327	WP_001052491.1	anti-sigma factor antagonist	-
GAU34_RS05195	1062674..1063153	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
GAU34_RS05200	1063128..1063898	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T149802 WP_000621175.1 NZ_CP048643:1060514-1060876 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T149802 NZ_CP064248:c92149-91733 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT149802 WP_000948331.1 NZ_CP048643:1060347-1060517 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT149802 NZ_CP064248:c92376-92146 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1