Detailed information of TA system TA149777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 683579..684219
• Replicon: chromosome
• Accession: NZ_CP048629
• Organism: Pseudomonas sp. OIL-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G3M63_RS03125
• Protein ID: WP_163981840.1
• Coordinates: 683809..684219 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G3M63_RS03120
• Protein ID: WP_163981839.1
• Coordinates: 683579..683809 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M63_RS03100	679035..679973	-	939	WP_150301759.1	hydroxymethylbilane synthase	-
G3M63_RS03105	680046..680795	-	750	WP_150301758.1	LytTR family DNA-binding domain-containing protein	-
G3M63_RS03110	680799..681896	-	1098	WP_150301757.1	histidine kinase	-
G3M63_RS03115	681979..683379	+	1401	WP_163981838.1	argininosuccinate lyase	-
G3M63_RS03120	683579..683809	+	231	WP_163981839.1	antitoxin	Antitoxin
G3M63_RS03125	683809..684219	+	411	WP_163981840.1	tRNA(fMet)-specific endonuclease VapC	Toxin
G3M63_RS03130	684285..685382	-	1098	WP_150301753.1	agmatine deiminase	-
G3M63_RS03135	685389..686267	-	879	WP_163981841.1	N-carbamoylputrescine amidase	-
G3M63_RS03140	686377..687726	-	1350	WP_163984165.1	lipopolysaccharide biosynthesis protein	-
G3M63_RS03145	687948..688799	-	852	WP_150301750.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15225.51 Da
• Isoelectric Point: 7.3228

>T149777 WP_163981840.1 NZ_CP048629:683809-684219 [Pseudomonas sp. OIL-1]
MLKFMLDTNICIFTIKNRPQSVREAFTQHHGQMCISTITLMELIYGAEKSSNPERNLAVIEGFATRLEVLSYDQAAAAHT
GQLRAELTKAGTPIGPYDQMIAGHARSQGLIVVTNNRREFERVPGLRIEDWIPPSI

Nucleotide

• Length: 411 bp

>T149777 NZ_CP064241:c4820164-4819880 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8598.55 Da
• Isoelectric Point: 4.4354

>AT149777 WP_163981839.1 NZ_CP048629:683579-683809 [Pseudomonas sp. OIL-1]
MEQGSVFQSNRSQAVRLPKAAALPDDVHRVDIVRLGRARLITPAGESWDSWFEGEGVTADFMIDREQPPEQEREAF

Nucleotide

• Length: 231 bp

>AT149777 NZ_CP064241:c4820396-4820154 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA