Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 855797..856448 Replicon chromosome
Accession NZ_CP048621
Organism Providencia stuartii strain MF1

Toxin (Protein)


Gene name Hha Uniprot ID K8W3H1
Locus tag G3A48_RS03670 Protein ID WP_004927064.1
Coordinates 855797..856000 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID B2Q721
Locus tag G3A48_RS03675 Protein ID WP_004927067.1
Coordinates 856080..856448 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3A48_RS03645 851676..852014 + 339 WP_004927053.1 P-II family nitrogen regulator -
G3A48_RS03650 852025..853308 + 1284 WP_014656764.1 ammonium transporter AmtB -
G3A48_RS03655 853545..854411 - 867 WP_004927058.1 acyl-CoA thioesterase II -
G3A48_RS03660 854733..855191 + 459 WP_004927062.1 YbaY family lipoprotein -
G3A48_RS03670 855797..856000 - 204 WP_004927064.1 hemolysin expression modulator Hha Toxin
G3A48_RS03675 856080..856448 - 369 WP_004927067.1 Hha toxicity modulator TomB Antitoxin
G3A48_RS03680 857016..858353 - 1338 WP_004927077.1 murein transglycosylase D -
G3A48_RS03685 858432..859187 - 756 WP_014656763.1 hydroxyacylglutathione hydrolase -
G3A48_RS03690 859221..859946 + 726 WP_053110025.1 methyltransferase domain-containing protein -
G3A48_RS03695 860025..860495 - 471 WP_104872987.1 ribonuclease HI -
G3A48_RS03700 860550..861311 + 762 WP_004927092.1 DNA polymerase III subunit epsilon -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8075.37 Da        Isoelectric Point: 6.9770

>T149774 WP_004927064.1 NZ_CP048621:c856000-855797 [Providencia stuartii]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSEDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Download         Length: 204 bp

>T149774 NZ_CP064241:c2742513-2741191 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14149.01 Da        Isoelectric Point: 4.2591

>AT149774 WP_004927067.1 NZ_CP048621:c856448-856080 [Providencia stuartii]
MDEYSPKNYDISELKYLCNSLNREAMLSLQKTNTHWVNDLSSPQSARLNELIEHIAAFVWQFKIKYPKENLVISLVEEYL
DETYDLFGSPVITLSEIIDWQSMNQNLVSVLDDDLKCPASKT

Download         Length: 369 bp

>AT149774 NZ_CP064241:c2742779-2742513 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8W3H1

Antitoxin

Source ID Structure
AlphaFold DB A0A140NKN2

References