Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1988..2535 Replicon plasmid unnamed3
Accession NZ_CP048612
Organism Escherichia coli strain STEFF_1

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag G3M66_RS25275 Protein ID WP_001384452.1
Coordinates 2257..2535 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID W0FTF6
Locus tag G3M66_RS25270 Protein ID WP_000079942.1
Coordinates 1988..2257 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M66_RS25265 (46) 46..1077 + 1032 WP_000907870.1 plasmid replication initiator RepA -
G3M66_RS25270 (1988) 1988..2257 + 270 WP_000079942.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
G3M66_RS25275 (2257) 2257..2535 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
G3M66_RS25280 (2581) 2581..2793 + 213 Protein_3 3'-5' exonuclease -
G3M66_RS25285 (3052) 3052..3981 - 930 WP_000102288.1 hypothetical protein -
G3M66_RS25290 (4418) 4418..5605 - 1188 WP_001697863.1 IS91 family transposase -
G3M66_RS25295 (5605) 5605..5970 - 366 WP_000124134.1 hypothetical protein -
G3M66_RS25300 (6118) 6118..7410 - 1293 Protein_7 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..88925 88925


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T149766 WP_001384452.1 NZ_CP048612:2257-2535 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T149766 NZ_CP064237:c162227-161952 [Klebsiella pneumoniae]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10282.61 Da        Isoelectric Point: 7.2057

>AT149766 WP_000079942.1 NZ_CP048612:1988-2257 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT149766 NZ_CP064237:c162511-162227 [Klebsiella pneumoniae]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A140JYS5

References