Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 32423..33024 Replicon plasmid unnamed2
Accession NZ_CP048611
Organism Escherichia coli strain STEFF_1

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag G3M66_RS24945 Protein ID WP_001216034.1
Coordinates 32644..33024 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag G3M66_RS24940 Protein ID WP_001190712.1
Coordinates 32423..32644 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M66_RS24900 (G3M66_24675) 27698..27886 + 189 WP_000797279.1 hypothetical protein -
G3M66_RS24905 27888..28066 + 179 Protein_33 hypothetical protein -
G3M66_RS24910 28400..28534 + 135 Protein_34 hypothetical protein -
G3M66_RS24915 (G3M66_24685) 28526..29410 + 885 WP_225902472.1 hypothetical protein -
G3M66_RS24920 (G3M66_24690) 29407..29769 + 363 WP_001261543.1 hypothetical protein -
G3M66_RS24925 (G3M66_24695) 30845..31201 - 357 WP_032272825.1 hypothetical protein -
G3M66_RS24930 (G3M66_24700) 31202..31786 - 585 WP_032272826.1 hypothetical protein -
G3M66_RS24935 (G3M66_24705) 31961..32350 + 390 WP_000506730.1 S24 family peptidase -
G3M66_RS24940 (G3M66_24710) 32423..32644 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
G3M66_RS24945 (G3M66_24715) 32644..33024 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
G3M66_RS24950 (G3M66_24720) 33029..33208 + 180 WP_000113019.1 hypothetical protein -
G3M66_RS24955 (G3M66_24725) 33236..34279 + 1044 WP_000648824.1 DUF968 domain-containing protein -
G3M66_RS24960 (G3M66_24730) 34368..34820 + 453 WP_001326849.1 late promoter-activating protein -
G3M66_RS24965 (G3M66_24735) 34906..36099 + 1194 WP_053890992.1 hypothetical protein -
G3M66_RS24970 (G3M66_24740) 36099..37583 + 1485 WP_000124150.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..95952 95952


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T149765 WP_001216034.1 NZ_CP048611:32644-33024 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T149765 NZ_CP064237:c101704-101555 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT149765 WP_001190712.1 NZ_CP048611:32423-32644 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT149765 NZ_CP064237:101749-101808 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References