Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 96626..97269 Replicon plasmid unnamed1
Accession NZ_CP048610
Organism Escherichia coli strain STEFF_1

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag G3M66_RS24620 Protein ID WP_001034044.1
Coordinates 96853..97269 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag G3M66_RS24615 Protein ID WP_001261286.1
Coordinates 96626..96856 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M66_RS24595 (93257) 93257..94012 - 756 WP_000852146.1 replication initiation protein RepE -
G3M66_RS24600 (94734) 94734..95540 - 807 WP_000016970.1 site-specific integrase -
G3M66_RS24605 (95541) 95541..95846 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
G3M66_RS24610 (95848) 95848..96066 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
G3M66_RS24615 (96626) 96626..96856 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
G3M66_RS24620 (96853) 96853..97269 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
G3M66_RS24625 (97344) 97344..98909 + 1566 WP_001128474.1 AAA family ATPase -
G3M66_RS24630 (98894) 98894..99916 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / sul3 / blaTEM-1B / aph(3')-Ia / aac(3)-IId / sitABCD iutA / iucD / iucC / iucB / iucA 1..119126 119126
- flank IS/Tn - - 100170..100673 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T149764 WP_001034044.1 NZ_CP048610:96853-97269 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T149764 NZ_CP064237:c49329-49171 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT149764 WP_001261286.1 NZ_CP048610:96626-96856 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT149764 NZ_CP064237:c49597-49373 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References