Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 95541..96066 Replicon plasmid unnamed1
Accession NZ_CP048610
Organism Escherichia coli strain STEFF_1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag G3M66_RS24605 Protein ID WP_001159871.1
Coordinates 95541..95846 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag G3M66_RS24610 Protein ID WP_000813630.1
Coordinates 95848..96066 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M66_RS24590 (91503) 91503..92669 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
G3M66_RS24595 (93257) 93257..94012 - 756 WP_000852146.1 replication initiation protein RepE -
G3M66_RS24600 (94734) 94734..95540 - 807 WP_000016970.1 site-specific integrase -
G3M66_RS24605 (95541) 95541..95846 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
G3M66_RS24610 (95848) 95848..96066 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
G3M66_RS24615 (96626) 96626..96856 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
G3M66_RS24620 (96853) 96853..97269 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
G3M66_RS24625 (97344) 97344..98909 + 1566 WP_001128474.1 AAA family ATPase -
G3M66_RS24630 (98894) 98894..99916 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / sul3 / blaTEM-1B / aph(3')-Ia / aac(3)-IId / sitABCD iutA / iucD / iucC / iucB / iucA 1..119126 119126
- flank IS/Tn - - 100170..100673 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T149763 WP_001159871.1 NZ_CP048610:c95846-95541 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T149763 NZ_CP064237:39900-40205 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT149763 WP_000813630.1 NZ_CP048610:c96066-95848 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT149763 NZ_CP064237:39680-39898 [Klebsiella pneumoniae]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References