Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4767984..4768600 Replicon chromosome
Accession NZ_CP048609
Organism Escherichia coli strain STEFF_1

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag G3M66_RS23005 Protein ID WP_001129490.1
Coordinates 4767984..4768358 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag G3M66_RS23010 Protein ID WP_001275523.1
Coordinates 4768358..4768600 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M66_RS22990 (4765487) 4765487..4766389 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
G3M66_RS22995 (4766386) 4766386..4767021 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
G3M66_RS23000 (4767018) 4767018..4767947 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
G3M66_RS23005 (4767984) 4767984..4768358 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
G3M66_RS23010 (4768358) 4768358..4768600 - 243 WP_001275523.1 CopG family transcriptional regulator Antitoxin
G3M66_RS23015 (4768820) 4768820..4769038 - 219 WP_001251290.1 CopG family transcriptional regulator -
G3M66_RS23020 (4769712) 4769712..4770626 - 915 WP_072649161.1 transposase -
G3M66_RS23025 (4770639) 4770639..4771526 - 888 Protein_4517 hypothetical protein -
G3M66_RS23030 (4771942) 4771942..4772883 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
G3M66_RS23035 (4772928) 4772928..4773365 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T149758 WP_001129490.1 NZ_CP048609:c4768358-4767984 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T149758 NZ_CP064235:c5399327-5398944 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT149758 WP_001275523.1 NZ_CP048609:c4768600-4768358 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT149758 NZ_CP064235:c5399569-5399327 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References