Detailed information of TA system TA149756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4350462..4351057
• Replicon: chromosome
• Accession: NZ_CP048609
• Organism: Escherichia coli strain STEFF_1

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: G3M66_RS21060
• Protein ID: WP_000239581.1
• Coordinates: 4350462..4350812 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: G3M66_RS21065
• Protein ID: WP_001223213.1
• Coordinates: 4350806..4351057 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M66_RS21040 (4345916)	4345916..4346938	-	1023	WP_001361374.1	ABC transporter permease	-
G3M66_RS21045 (4346952)	4346952..4348454	-	1503	WP_001095661.1	ATP-binding cassette domain-containing protein	-
G3M66_RS21050 (4348587)	4348587..4349543	-	957	WP_108997067.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
G3M66_RS21055 (4349853)	4349853..4350383	+	531	WP_000055072.1	inorganic diphosphatase	-
G3M66_RS21060 (4350462)	4350462..4350812	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
G3M66_RS21065 (4350806)	4350806..4351057	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
G3M66_RS21070 (4351269)	4351269..4351610	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
G3M66_RS21075 (4351613)	4351613..4355392	-	3780	WP_108997070.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T149756 WP_000239581.1 NZ_CP048609:c4350812-4350462 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T149756 NZ_CP064235:c4918993-4918709 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT149756 WP_001223213.1 NZ_CP048609:c4351057-4350806 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT149756 NZ_CP064235:c4919225-4918983 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7