Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4244250..4244826 Replicon chromosome
Accession NZ_CP048609
Organism Escherichia coli strain STEFF_1

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag G3M66_RS20580 Protein ID WP_001131965.1
Coordinates 4244539..4244826 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag G3M66_RS20575 Protein ID WP_000063158.1
Coordinates 4244250..4244552 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M66_RS20555 (4239358) 4239358..4240578 + 1221 WP_000873756.1 amino acid deaminase -
G3M66_RS20560 (4240846) 4240846..4242996 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
G3M66_RS20565 (4243046) 4243046..4243249 + 204 WP_000467859.1 YbdD/YjiX family protein -
G3M66_RS20570 (4243260) 4243260..4244216 + 957 WP_001361361.1 GTPase -
G3M66_RS20575 (4244250) 4244250..4244552 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
G3M66_RS20580 (4244539) 4244539..4244826 - 288 WP_001131965.1 BrnT family toxin Toxin
G3M66_RS20585 (4245023) 4245023..4246189 + 1167 WP_000800823.1 restriction endonuclease -
G3M66_RS20590 (4246234) 4246234..4246407 + 174 Protein_4046 type I restriction-modification system subunit M N-terminal domain-containing protein -
G3M66_RS20595 (4246401) 4246401..4247069 + 669 Protein_4047 DUF3387 domain-containing protein -
G3M66_RS20600 (4247284) 4247284..4247622 + 339 WP_000132633.1 endoribonuclease SymE -
G3M66_RS20605 (4247710) 4247710..4247757 - 48 WP_229017048.1 hypothetical protein -
G3M66_RS20610 (4248005) 4248005..4249417 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T149754 WP_001131965.1 NZ_CP048609:c4244826-4244539 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T149754 NZ_CP064235:4203124-4203342 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT149754 WP_000063158.1 NZ_CP048609:c4244552-4244250 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT149754 NZ_CP064235:4202723-4203097 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References