Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3670553..3671171 Replicon chromosome
Accession NZ_CP048609
Organism Escherichia coli strain STEFF_1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag G3M66_RS17760 Protein ID WP_001291435.1
Coordinates 3670953..3671171 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag G3M66_RS17755 Protein ID WP_000344800.1
Coordinates 3670553..3670927 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M66_RS17745 (3665642) 3665642..3666835 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
G3M66_RS17750 (3666858) 3666858..3670007 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
G3M66_RS17755 (3670553) 3670553..3670927 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
G3M66_RS17760 (3670953) 3670953..3671171 + 219 WP_001291435.1 HHA domain-containing protein Toxin
G3M66_RS17765 (3671343) 3671343..3671894 + 552 WP_000102574.1 maltose O-acetyltransferase -
G3M66_RS17770 (3672010) 3672010..3672480 + 471 WP_000136192.1 YlaC family protein -
G3M66_RS17775 (3672644) 3672644..3674194 + 1551 WP_001361689.1 cyclic-guanylate-specific phosphodiesterase PdeB -
G3M66_RS17780 (3674236) 3674236..3674589 - 354 WP_000878140.1 DUF1428 domain-containing protein -
G3M66_RS17790 (3674968) 3674968..3675279 + 312 WP_000409911.1 MGMT family protein -
G3M66_RS17795 (3675310) 3675310..3675882 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149752 WP_001291435.1 NZ_CP048609:3670953-3671171 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T149752 NZ_CP064235:2010498-2010600 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT149752 WP_000344800.1 NZ_CP048609:3670553-3670927 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT149752 NZ_CP064235:c2010607-2010462 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References