Detailed information of TA system TA149750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3245631..3246336
• Replicon: chromosome
• Accession: NZ_CP048609
• Organism: Escherichia coli strain STEFF_1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: G3M66_RS15650
• Protein ID: WP_000539524.1
• Coordinates: 3245631..3246017 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: G3M66_RS15655
• Protein ID: WP_001280945.1
• Coordinates: 3246007..3246336 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M66_RS15630 (3241635)	3241635..3242261	+	627	WP_001295292.1	glutathione S-transferase GstB	-
G3M66_RS15635 (3242258)	3242258..3243373	-	1116	WP_000555056.1	aldose sugar dehydrogenase YliI	-
G3M66_RS15640 (3243484)	3243484..3243867	-	384	WP_000497137.1	biofilm formation regulator BssR	-
G3M66_RS15645 (3244080)	3244080..3245405	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
G3M66_RS15650 (3245631)	3245631..3246017	+	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G3M66_RS15655 (3246007)	3246007..3246336	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
G3M66_RS15660 (3246406)	3246406..3247723	-	1318	Protein_3085	diguanylate cyclase	-
G3M66_RS15665 (3247731)	3247731..3250079	-	2349	WP_000950331.1	EAL domain-containing protein	-
G3M66_RS15670 (3250256)	3250256..3251167	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T149750 WP_000539524.1 NZ_CP048609:3245631-3246017 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T149750 NZ_CP064235:745207-745692 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT149750 WP_001280945.1 NZ_CP048609:3246007-3246336 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT149750 NZ_CP064235:744917-745210 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7