Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 350936..351567 Replicon chromosome
Accession NZ_CP048609
Organism Escherichia coli strain STEFF_1

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag G3M66_RS01595 Protein ID WP_001260301.1
Coordinates 350936..351211 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag G3M66_RS01600 Protein ID WP_000593555.1
Coordinates 351208..351567 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M66_RS01580 345940..347007 + 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -
G3M66_RS01585 347004..349739 + 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
G3M66_RS01590 349739..350863 + 1125 WP_001314210.1 ABC transporter permease -
G3M66_RS01595 350936..351211 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
G3M66_RS01600 351208..351567 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
G3M66_RS01605 351687..352088 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
G3M66_RS01610 352093..352899 - 807 WP_061092823.1 nickel import ATP-binding protein NikE -
G3M66_RS01615 352896..353660 - 765 WP_001136211.1 nickel import ATP-binding protein NikD -
G3M66_RS01620 353660..354493 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
G3M66_RS01625 354490..355434 - 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 343952..352088 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T149734 WP_001260301.1 NZ_CP048609:350936-351211 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T149734 NZ_CP064232:327907-328275 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT149734 WP_000593555.1 NZ_CP048609:351208-351567 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT149734 NZ_CP064232:327689-327910 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References