Detailed information of TA system TA149728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 4220469..4221052
• Replicon: chromosome
• Accession: NZ_CP048605
• Organism: Escherichia coli strain PapRG-06-5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G3M73_RS19740
• Protein ID: WP_001625796.1
• Coordinates: 4220717..4221052 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: U9Y5F1
• Locus tag: G3M73_RS19735
• Protein ID: WP_000581936.1
• Coordinates: 4220469..4220717 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M73_RS19725	4216808..4218109	+	1302	WP_021557151.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
G3M73_RS19730	4218157..4220391	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
G3M73_RS19735	4220469..4220717	+	249	WP_000581936.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
G3M73_RS19740	4220717..4221052	+	336	WP_001625796.1	endoribonuclease MazF	Toxin
G3M73_RS19745	4221123..4221914	+	792	WP_001071643.1	nucleoside triphosphate pyrophosphohydrolase	-
G3M73_RS19750	4222142..4223779	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
G3M73_RS19755	4223867..4225165	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12128.07 Da
• Isoelectric Point: 8.2618

>T149728 WP_001625796.1 NZ_CP048605:4220717-4221052 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALTDQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T149728 NZ_CP064229:c5327929-5327546 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9341.71 Da
• Isoelectric Point: 4.4536

>AT149728 WP_000581936.1 NZ_CP048605:4220469-4220717 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHDNIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT149728 NZ_CP064229:c5328171-5327929 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: U9Y5F1