Detailed information of TA system TA149725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3531420..3532174
• Replicon: chromosome
• Accession: NZ_CP048605
• Organism: Escherichia coli strain PapRG-06-5

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: G3M73_RS16360
• Protein ID: WP_134873432.1
• Coordinates: 3531420..3531905 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: G3M73_RS16365
• Protein ID: WP_000801914.1
• Coordinates: 3531896..3532174 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M73_RS16340	3526587..3527345	+	759	WP_000815099.1	DeoR/GlpR family transcriptional regulator	-
G3M73_RS16345	3527327..3528925	-	1599	WP_089628895.1	DNA-binding transcriptional regulator RtcR	-
G3M73_RS16350	3529112..3530338	+	1227	WP_163425368.1	RNA-splicing ligase RtcB	-
G3M73_RS16355	3530342..3531370	+	1029	WP_021574798.1	RNA 3'-terminal phosphate cyclase	-
G3M73_RS16360	3531420..3531905	-	486	WP_134873432.1	GNAT family N-acetyltransferase	Toxin
G3M73_RS16365	3531896..3532174	-	279	WP_000801914.1	DUF1778 domain-containing protein	Antitoxin
G3M73_RS16370	3532241..3534946	-	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17681.59 Da
• Isoelectric Point: 9.4066

>T149725 WP_134873432.1 NZ_CP048605:c3531905-3531420 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLQYLITL
E

Nucleotide

• Length: 486 bp

>T149725 NZ_CP064229:4098060-4098278 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10406.91 Da
• Isoelectric Point: 4.6336

>AT149725 WP_000801914.1 NZ_CP048605:c3532174-3531896 [Escherichia coli]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFIVDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT149725 NZ_CP064229:4097659-4098033 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG