Detailed information of TA system TA149721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2574930..2575525
• Replicon: chromosome
• Accession: NZ_CP048605
• Organism: Escherichia coli strain PapRG-06-5

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: A0A8S7NE19
• Locus tag: G3M73_RS11995
• Protein ID: WP_044189483.1
• Coordinates: 2574930..2575280 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: G3M73_RS12000
• Protein ID: WP_001223208.1
• Coordinates: 2575274..2575525 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M73_RS11975	2570376..2571398	-	1023	WP_088375740.1	ABC transporter permease	-
G3M73_RS11980	2571412..2572914	-	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
G3M73_RS11985	2573054..2574010	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
G3M73_RS11990	2574320..2574850	+	531	WP_000055075.1	inorganic diphosphatase	-
G3M73_RS11995	2574930..2575280	-	351	WP_044189483.1	endoribonuclease toxin ChpB	Toxin
G3M73_RS12000	2575274..2575525	-	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
G3M73_RS12005	2575737..2576078	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
G3M73_RS12010	2576081..2579860	-	3780	WP_163425267.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-115)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12541.47 Da
• Isoelectric Point: 5.6219

>T149721 WP_044189483.1 NZ_CP048605:c2575280-2574930 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASSHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T149721 NZ_CP064229:732175-732660 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT149721 WP_001223208.1 NZ_CP048605:c2575525-2575274 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT149721 NZ_CP064229:731885-732178 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26