Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2436170..2436746 Replicon chromosome
Accession NZ_CP048605
Organism Escherichia coli strain PapRG-06-5

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag G3M73_RS11490 Protein ID WP_001299718.1
Coordinates 2436459..2436746 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A7U9DAW4
Locus tag G3M73_RS11485 Protein ID WP_000063150.1
Coordinates 2436170..2436472 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M73_RS11465 2431780..2432292 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
G3M73_RS11470 2432685..2434835 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
G3M73_RS11475 2434966..2435169 + 204 WP_000467859.1 YbdD/YjiX family protein -
G3M73_RS11480 2435180..2436136 + 957 WP_086259868.1 GTPase -
G3M73_RS11485 2436170..2436472 - 303 WP_000063150.1 BrnA antitoxin family protein Antitoxin
G3M73_RS11490 2436459..2436746 - 288 WP_001299718.1 BrnT family toxin Toxin
G3M73_RS11495 2436901..2437635 - 735 WP_000208603.1 hypothetical protein -
G3M73_RS11500 2437679..2439778 - 2100 WP_000648213.1 DUF262 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T149720 WP_001299718.1 NZ_CP048605:c2436746-2436459 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T149720 NZ_CP064229:327907-328275 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11532.03 Da        Isoelectric Point: 9.4646

>AT149720 WP_000063150.1 NZ_CP048605:c2436472-2436170 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT149720 NZ_CP064229:327689-327910 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DAW4

References