Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2316807..2317357 Replicon chromosome
Accession NZ_CP048605
Organism Escherichia coli strain PapRG-06-5

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag G3M73_RS10920 Protein ID WP_001160974.1
Coordinates 2316807..2317121 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag G3M73_RS10925 Protein ID WP_000125566.1
Coordinates 2317124..2317357 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M73_RS10895 2312482..2313768 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
G3M73_RS10900 2313768..2314757 + 990 WP_062882910.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
G3M73_RS10905 2314754..2315575 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
G3M73_RS10910 2315578..2315955 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
G3M73_RS10915 2315962..2316810 + 849 WP_160412351.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
G3M73_RS10920 2316807..2317121 - 315 WP_001160974.1 CcdB family protein Toxin
G3M73_RS10925 2317124..2317357 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
G3M73_RS10930 2317443..2317922 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
G3M73_RS10935 2318114..2319976 - 1863 WP_000377191.1 glutathione-regulated potassium-efflux system protein KefC -
G3M73_RS10940 2319969..2320499 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
G3M73_RS10945 2320607..2321938 - 1332 WP_001183198.1 MFS transporter -
G3M73_RS10950 2321996..2322283 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T149719 WP_001160974.1 NZ_CP048605:c2317121-2316807 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T149719 NZ_CP064228:c483-217 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT149719 WP_000125566.1 NZ_CP048605:c2317357-2317124 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT149719 NZ_CP064228:c691-467 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References