149715

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1831703..1832540 Replicon chromosome
Accession NZ_CP048605
Organism Escherichia coli strain PapRG-06-5

Toxin (Protein)


Gene name itaT Uniprot ID A0A080JA52
Locus tag G3M73_RS08660 Protein ID WP_032170811.1
Coordinates 1831998..1832540 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A0A080JFU1
Locus tag G3M73_RS08655 Protein ID WP_001577859.1
Coordinates 1831703..1832014 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M73_RS08630 1826723..1827670 + 948 WP_001239434.1 cytochrome o ubiquinol oxidase subunit II -
G3M73_RS08635 1827692..1829683 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
G3M73_RS08640 1829673..1830287 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
G3M73_RS08645 1830287..1830616 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
G3M73_RS08650 1830628..1831518 + 891 WP_000971336.1 heme o synthase -
G3M73_RS08655 1831703..1832014 + 312 WP_001577859.1 DUF1778 domain-containing protein Antitoxin
G3M73_RS08660 1831998..1832540 + 543 WP_032170811.1 GNAT family N-acetyltransferase Toxin
G3M73_RS08665 1832596..1833531 - 936 WP_163425146.1 sel1 repeat family protein -
G3M73_RS08670 1833937..1835301 + 1365 WP_001000956.1 MFS transporter -
G3M73_RS08675 1835429..1835920 - 492 WP_001138904.1 nucleotide binding protein YajQ -
G3M73_RS08680 1836088..1836999 + 912 WP_000705865.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19835.05 Da        Isoelectric Point: 8.3395

>T149715 WP_032170811.1 NZ_CP048605:1831998-1832540 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRTSLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T149715 NZ_CP064225:67875-68033 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11400.08 Da        Isoelectric Point: 10.5548

>AT149715 WP_001577859.1 NZ_CP048605:1831703-1832014 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNRLREAAQAVGLDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT149715 NZ_CP064225:67764-67829 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A080JA52

Antitoxin

Source ID Structure
AlphaFold DB A0A080JFU1

References