Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1797613..1798231 Replicon chromosome
Accession NZ_CP048605
Organism Escherichia coli strain PapRG-06-5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag G3M73_RS08490 Protein ID WP_001291435.1
Coordinates 1798013..1798231 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag G3M73_RS08485 Protein ID WP_000344800.1
Coordinates 1797613..1797987 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M73_RS08475 1792702..1793895 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
G3M73_RS08480 1793918..1797067 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
G3M73_RS08485 1797613..1797987 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
G3M73_RS08490 1798013..1798231 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
G3M73_RS08495 1798403..1798954 + 552 WP_000102564.1 maltose O-acetyltransferase -
G3M73_RS08500 1799070..1799540 + 471 WP_000136192.1 YlaC family protein -
G3M73_RS08505 1799704..1801254 + 1551 WP_024210825.1 cyclic-guanylate-specific phosphodiesterase PdeB -
G3M73_RS08510 1801296..1801649 - 354 WP_000878140.1 DUF1428 domain-containing protein -
G3M73_RS08520 1802028..1802339 + 312 WP_163425142.1 MGMT family protein -
G3M73_RS08525 1802370..1802942 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149714 WP_001291435.1 NZ_CP048605:1798013-1798231 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T149714 NZ_CP064225:43518-43823 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT149714 WP_000344800.1 NZ_CP048605:1797613-1797987 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT149714 NZ_CP064225:43298-43516 [Klebsiella pneumoniae]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References