Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 688682..689320 Replicon chromosome
Accession NZ_CP048605
Organism Escherichia coli strain PapRG-06-5

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag G3M73_RS03320 Protein ID WP_000813794.1
Coordinates 689144..689320 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag G3M73_RS03315 Protein ID WP_001270286.1
Coordinates 688682..689098 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M73_RS03295 683834..684775 - 942 WP_160438281.1 ABC transporter permease -
G3M73_RS03300 684776..685789 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
G3M73_RS03305 685807..686952 - 1146 WP_001773579.1 ABC transporter substrate-binding protein -
G3M73_RS03310 687197..688603 - 1407 WP_163424996.1 PLP-dependent aminotransferase family protein -
G3M73_RS03315 688682..689098 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
G3M73_RS03320 689144..689320 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
G3M73_RS03325 689542..689772 + 231 WP_072058135.1 YncJ family protein -
G3M73_RS03330 689864..691825 - 1962 WP_163424997.1 23S rRNA 5-hydroxycytidine C2501 synthase -
G3M73_RS03335 691898..692434 - 537 WP_021577428.1 DNA-binding transcriptional regulator SutR -
G3M73_RS03340 692487..693698 + 1212 WP_163424998.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T149712 WP_000813794.1 NZ_CP048605:c689320-689144 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T149712 NZ_CP064224:118883-119194 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT149712 WP_001270286.1 NZ_CP048605:c689098-688682 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT149712 NZ_CP064224:119191-119610 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References