Detailed information of TA system TA149702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4299346..4299971
• Replicon: chromosome
• Accession: NZ_CP048604
• Organism: Escherichia coli strain PapRG-06-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G3M72_RS20500
• Protein ID: WP_000911330.1
• Coordinates: 4299573..4299971 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: G3M72_RS20495
• Protein ID: WP_000450524.1
• Coordinates: 4299346..4299573 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M72_RS20470	4295149..4295619	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
G3M72_RS20475	4295619..4296191	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
G3M72_RS20480	4296337..4297215	+	879	WP_001345767.1	4-hydroxy-tetrahydrodipicolinate synthase	-
G3M72_RS20485	4297232..4298266	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
G3M72_RS20490	4298479..4299192	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
G3M72_RS20495	4299346..4299573	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
G3M72_RS20500	4299573..4299971	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
G3M72_RS20505	4300118..4300981	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
G3M72_RS20510	4300996..4303011	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
G3M72_RS20515	4303085..4303783	+	699	WP_000679812.1	esterase	-
G3M72_RS20520	4303893..4304093	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T149702 WP_000911330.1 NZ_CP048604:4299573-4299971 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T149702 NZ_CP064223:878079-878486 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT149702 WP_000450524.1 NZ_CP048604:4299346-4299573 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT149702 NZ_CP064223:877832-878098 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33