Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3861064..3861718 Replicon chromosome
Accession NZ_CP048604
Organism Escherichia coli strain PapRG-06-3

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag G3M72_RS18430 Protein ID WP_000244772.1
Coordinates 3861311..3861718 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G3M72_RS18425 Protein ID WP_000354046.1
Coordinates 3861064..3861330 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M72_RS18405 3857351..3857662 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
G3M72_RS18410 3857826..3858485 + 660 WP_000250274.1 hemolysin III family protein -
G3M72_RS18415 3858608..3859588 - 981 WP_000399647.1 IS110-like element IS621 family transposase -
G3M72_RS18420 3859841..3860821 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
G3M72_RS18425 3861064..3861330 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G3M72_RS18430 3861311..3861718 + 408 WP_000244772.1 protein YgfX Toxin
G3M72_RS18435 3861758..3862279 - 522 WP_001055874.1 flavodoxin FldB -
G3M72_RS18440 3862391..3863287 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G3M72_RS18445 3863312..3864022 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G3M72_RS18450 3864028..3865761 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3858608..3859588 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T149700 WP_000244772.1 NZ_CP048604:3861311-3861718 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T149700 NZ_CP064223:355415-355762 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT149700 WP_000354046.1 NZ_CP048604:3861064-3861330 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT149700 NZ_CP064223:355762-356061 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References