Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4256973..4257470 Replicon chromosome
Accession NZ_CP048603
Organism Escherichia coli strain ChlosBP-23-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G3M71_RS20380 Protein ID WP_001173343.1
Coordinates 4256973..4257146 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag G3M71_RS20385 Protein ID WP_001312331.1
Coordinates 4257174..4257470 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M71_RS20370 4253643..4255100 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
G3M71_RS20375 4255337..4256854 + 1518 WP_000003805.1 lysine--tRNA ligase -
G3M71_RS20380 4256973..4257146 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G3M71_RS20385 4257174..4257470 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G3M71_RS20390 4257698..4257970 - 273 WP_000405647.1 N-acetyltransferase -
G3M71_RS20395 4257982..4258212 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
G3M71_RS20400 4258393..4260024 + 1632 WP_163388771.1 sensor histidine kinase -
G3M71_RS20405 4260021..4260740 + 720 WP_163388772.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - papD / papC 4198179..4257470 59291


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T149682 WP_001173343.1 NZ_CP048603:c4257146-4256973 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T149682 NZ_CP064217:2000363-2000465 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT149682 WP_001312331.1 NZ_CP048603:c4257470-4257174 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT149682 NZ_CP064217:c2000472-2000327 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References